Mosca A, Carpinelli A, Bonini P A
Dipartimento di Scienze e Tecnologie Biomediche, Universita' degli Studi di Milano, Italy.
J Clin Chem Clin Biochem. 1987 Jul;25(7):447-50.
The direct effect of the ethylene glycol on the quantitation of glycated haemoglobins by ion-exchange minicolumn chromatography, affinity chromatography and high performance liquid chromatography was investigated. No evident effect was found for ethylene glycol concentrations between 0.0 and 0.5 (volume fraction). The ethylene glycol-stabilised haemolysates were also analysed by HPLC: the elution profiles were similar to those of haemolysates fresly prepared from whole blood, and they remained unchanged, even after 18 months of storage at -20 degrees C. A preliminary inter-laboratory trial among 15 Italian laboratories was organized, using these ethylene glycol-stabilised haemolysates and, for comparison, a lyophilized commercial preparation. From the data obtained in this trial we confirmed the validity of using ethylene glycol-stabilized haemolysates as control materials, both for intra- and inter-laboratory standardization of glycated haemoglobin measurements.
研究了乙二醇对离子交换微柱色谱法、亲和色谱法和高效液相色谱法定量糖化血红蛋白的直接影响。在乙二醇浓度为0.0至0.5（体积分数）之间未发现明显影响。还用高效液相色谱法分析了乙二醇稳定的溶血产物：洗脱图谱与从全血新鲜制备的溶血产物相似，即使在-20℃下储存18个月后仍保持不变。在15个意大利实验室之间组织了一项初步的实验室间试验，使用这些乙二醇稳定的溶血产物，并作为比较，使用了一种冻干的商业制剂。从该试验获得的数据中，我们证实了使用乙二醇稳定的溶血产物作为对照材料用于糖化血红蛋白测量的实验室内和实验室间标准化的有效性。
