De Felici M, Dolci S, Siracusa G
Department of Anatomy and Cell Biology, II University of Rome, Italy.
J Exp Zool. 1987 Aug;243(2):283-7. doi: 10.1002/jez.1402430213.
The lethality caused by calcium-free medium (CFM) to fully grown mouse oocytes significantly decreases if a disulfide reducing agent (dithiothreitol, reduced glutathione, or L-cysteine) is added to the medium. In this condition, most of the surviving oocytes do not spontaneously resume meiosis. We also show that the sulfhydryl content of fully grown oocytes, estimated by monobromobimane labeling, rapidly decreases during culture in CFM. The hypothesis is discussed that lethality of oocytes cultured in CFM may be a consequence of an alteration of thiol-disulfide balance.
如果向无钙培养基（CFM）中添加二硫键还原剂（二硫苏糖醇、还原型谷胱甘肽或L-半胱氨酸），其对完全成熟小鼠卵母细胞造成的致死率会显著降低。在此条件下，大多数存活的卵母细胞不会自发恢复减数分裂。我们还表明，通过单溴代双马来酰亚胺标记估算，完全成熟卵母细胞的巯基含量在CFM培养过程中会迅速降低。本文讨论了一个假说，即在CFM中培养的卵母细胞的致死率可能是硫醇-二硫键平衡改变的结果。
