Heidemann S R, Hamborg M A
J Exp Zool. 1984 Jul;231(1):93-100. doi: 10.1002/jez.1402310112.
We measured several of the components involved in the thiol-disulfide balance of immature and mature oocytes of Xenopus laevis: total soluble native sulfhydryl, reduced and oxidized glutathione, native sulfhydryl content of soluble protein, and disulfide content of soluble protein. None of these values changed during maturation. Both the immature and mature oocyte were characterized by a relatively high disulfide to sulfhydryl ratio. We also measured native sulfhydryl content of germinal vesicle proteins and enucleated oocyte proteins in immature oocytes. Contrary to early cytochemical findings, our measurements suggest that the germinal vesicle has a lower concentration of protein sulfhydryl than does the cytoplasm.
我们测定了非洲爪蟾未成熟和成熟卵母细胞中硫醇 - 二硫键平衡所涉及的几个组分：总可溶性天然巯基、还原型和氧化型谷胱甘肽、可溶性蛋白质的天然巯基含量以及可溶性蛋白质的二硫键含量。在成熟过程中，这些值均未发生变化。未成熟和成熟卵母细胞均具有相对较高的二硫键与巯基比率。我们还测定了未成熟卵母细胞中生发泡蛋白和去核卵母细胞蛋白的天然巯基含量。与早期细胞化学研究结果相反，我们的测量结果表明，生发泡中蛋白质巯基的浓度低于细胞质中的浓度。
