Ganesan Raja, Mukherjee Anirban Goutam, Gopalakrishnan Abilash Valsala, Prabhakaran Vasantha-Srinivasan
Institute for Liver and Digestive Diseases, College of Medicine, Hallym University, Chuncheon 24253, Republic of Korea.
Department of Biological Sciences, Pusan National University, Busan 46241, Republic of Korea.
Metabolites. 2022 Dec 14;12(12):1263. doi: 10.3390/metabo12121263.
In this study, we demonstrated that chitosan-applied zebrafish () tissue metabolite alteration, metabolic discrimination, and metabolic phenotypic expression occurred. The spectroscopy of solid-state H nuclear magnetic resonance (ss H-NMR) has been used. Chitosan has no, or low, toxicity and is a biocompatible biomaterial; however, the metabolite mechanisms underlying the biological effect of chitosan are poorly understood. The zebrafish is now one of the most popular ecotoxicology models. Zebrafish were exposed to chitosan concentrations of 0, 50, 100, 200, and 500 mg/L, and the body tissue was subjected to metabolites-targeted profiling. The zebrafish samples were measured via solvent-suppressed and T-filtered methods with in vivo zebrafish metabolites. The metabolism of glutamate, glutamine, glutathione (GSH), taurine, trimethylamine (TMA), and its N-oxide (TMAO) is also significantly altered. Here, we report the quantification of metabolites and the biological application of chitosan. The metabolomics profile of chitosan in zebrafish has been detected, and the results indicated disturbed amino acid metabolism, the TCA cycle, and glycolysis. Our results demonstrate the potential of comparative metabolite profiling for discovering bioactive metabolites and they highlight the power of chitosan-applied chemical metabolomics to uncover new biological insights.
在本研究中，我们证明了施用壳聚糖的斑马鱼出现了组织代谢物改变、代谢辨别和代谢表型表达。我们使用了固态氢核磁共振（ss H-NMR）光谱法。壳聚糖无毒或低毒，是一种生物相容性生物材料；然而，壳聚糖生物效应背后的代谢物机制却知之甚少。斑马鱼是目前最受欢迎的生态毒理学模型之一。将斑马鱼暴露于浓度为0、50、100、200和500 mg/L的壳聚糖中，并对其身体组织进行代谢物靶向分析。通过溶剂抑制和T滤波方法对斑马鱼样本与体内斑马鱼代谢物进行测量。谷氨酸、谷氨酰胺、谷胱甘肽（GSH）、牛磺酸、三甲胺（TMA）及其N-氧化物（TMAO）的代谢也发生了显著改变。在此，我们报告了代谢物的定量分析以及壳聚糖的生物学应用。已检测到斑马鱼中壳聚糖的代谢组学图谱，结果表明氨基酸代谢、三羧酸循环和糖酵解受到干扰。我们的结果证明了比较代谢物分析在发现生物活性代谢物方面的潜力，并突出了施用壳聚糖的化学代谢组学在揭示新的生物学见解方面的作用。
