Research Group on Therapeutic Strategies, Institute of Chemistry and Biotechnology, Federal University of Alagoas, Maceió, AL, Brazil.
Research Group on Therapeutic Strategies, Institute of Chemistry and Biotechnology, Federal University of Alagoas, Maceió, AL, Brazil.
Sci Total Environ. 2023 Apr 10;868:161737. doi: 10.1016/j.scitotenv.2023.161737. Epub 2023 Jan 21.
Metabolomics is an innovative approach used in the medical, toxicological, and biological sciences. As an interdisciplinary topic, metabolomics and its relation with the environment and toxicological research are extensive. The use of substances, such as drugs and pesticides, contributes to the continuous releasing of xenobiotics into the environment, harming organisms and their habitats. In this context, fish are important bioindicators of the environmental condition and have often been used as model species. Among them, zebrafish (Danio rerio) presents itself as a versatile and straightforward option due to its unique attributes for research. Zebrafish proves to be a valuable model for toxicity assays and also for metabolomics profiling by analytical tools. Thus, NMR-based metabolomics associated with statistical analysis can reasonably assist researchers in critical factors related to discovering and validating biomarkers through accurate diagnosis. Therefore, this review aimed to report the studies that applied zebrafish as a model for (eco)toxicological assays and essentially utilized NMR-based metabolomics analysis to assess the biochemical profile and thus suggest the potential biological marker.
代谢组学是一种应用于医学、毒理学和生物学科学的创新方法。作为一个跨学科的主题，代谢组学及其与环境和毒理学研究的关系非常广泛。物质的使用，如药物和农药，导致不断向环境中释放外来物质，危害生物及其栖息地。在这种情况下，鱼类是环境状况的重要生物指标，并且经常被用作模式生物。其中，斑马鱼（Danio rerio）因其独特的研究属性而成为一种多功能且简单的选择。斑马鱼被证明是毒性测定和分析工具代谢组学分析的有价值的模型。因此，基于 NMR 的代谢组学与统计分析相结合，可以合理地帮助研究人员发现和验证生物标志物的关键因素，通过准确诊断。因此，本综述旨在报告应用斑马鱼作为（生态）毒理学测定模型的研究，并主要利用基于 NMR 的代谢组学分析来评估生化特征，从而提出潜在的生物标志物。
