Pinto Ana A, Ruano-González Antonio, Ezzanad Abdellah, Pinedo-Rivilla Cristina, Sánchez-Maestre Rosario, Amaro-Luis Juan Manuel
Departamento de Química Orgánica, Facultad de Ciencias, Universidad de Cádiz, Campus Rio San Pedro, 11510 Puerto Real, Spain.
Laboratorio de Productos Naturales, Departamento de Química, Facultad de Ciencias, Universidad de Los Andes (ULA), Mérida C.P. 5101, Venezuela.
Metabolites. 2022 Dec 19;12(12):1292. doi: 10.3390/metabo12121292.
genus is widely used in traditional treatment against fever, headache, hepatobiliary disorders, skin ulcers, diabetes, and rheumatism, as well as an antispasmodic and diuretic. Its phytochemistry mainly shows the presence of flavonoids and terpenoids such as monoterpenes, sesquiterpenes, diterpenes, and triterpenes. Some of them have been evaluated for biological activities presenting allelopathic, antimicrobial, cytotoxic, and anti-inflammatory properties. In this paper, our research group reported the isolation, characterization, and antifungal evaluation of several molecules isolated from the dichloromethane extract from , , and against the phytopathogen fungus . The isolated compounds have not previously been tested against , revealing an important source of antifungals in the genus . Six known flavones were isolated from . The dichloromethane extracts of and were subjected to a bio-guided isolation, obtaining three known flavones, an α-hydroxidihydrochalcone mixture, one labdane, one triterpene, and two norbisabolenes from the most active fractions. The compounds 4'-methoxy-α-hydroxydihydrochalcone (), 3β,15-dihydroxylabdan-7-en-17-al (), and 13-nor-11,12-dihydroxybisabol-2-enone () are novel. The most active compounds were the Salvigenin () and 1,2-dihydrosenedigital-2-one () with an IC50 of 13.5 and 3.1 μg/mL, respectively.
该属植物在传统治疗中被广泛用于对抗发烧、头痛、肝胆疾病、皮肤溃疡、糖尿病和风湿病，还具有解痉和利尿作用。其植物化学主要表现为存在黄酮类化合物和萜类化合物，如单萜、倍半萜、二萜和三萜。其中一些已被评估具有化感、抗菌、细胞毒性和抗炎特性等生物活性。在本文中，我们的研究小组报告了从[植物名称1]、[植物名称2]和[植物名称3]的二氯甲烷提取物中分离得到的几种分子对植物病原真菌[真菌名称]的分离、表征及抗真菌评估。这些分离出的化合物此前未针对[真菌名称]进行过测试，揭示了该属植物中一个重要的抗真菌来源。从[植物名称1]中分离出了六种已知的黄酮。对[植物名称2]和[植物名称3]的二氯甲烷提取物进行了生物导向分离，从活性最高的馏分中获得了三种已知的黄酮、一种α-羟基二氢查耳酮混合物、一种半日花烷、一种三萜和两种降荜澄茄烯。化合物4'-甲氧基-α-羟基二氢查耳酮（[化合物1名称]）、3β,15-二羟基半日花-7-烯-17-醛（[化合物2名称]）和13-降-11,12-二羟基荜澄茄-2-烯酮（[化合物3名称]）是新化合物。活性最高的化合物是柳穿鱼黄素（[化合物4名称]）和1,2-二氢洋地黄毒苷-2-酮（[化合物5名称]），其IC50分别为13.5和3.1μg/mL。
