从 Kunth 中分离的萜类和类黄酮对 TPA 诱导的小鼠耳水肿的影响。
Effect of Terpenoids and Flavonoids Isolated from Kunth on TPA-Induced Ear Edema in Mice.
机构信息
Centro de Desarrollo de Productos Bióticos. Instituto Politécnico Nacional (IPN), Col. San Isidro, Carretera Yautepec-Jojutla, Km 6, 62731, Morelos, Mexico.
Centro de Investigación Biomédica del Sur, Instituto Mexicano del Seguro Social, Argentina 1, Col. Centro, Xochitepec, 62790 Morelos, Mexico.
出版信息
Molecules. 2020 Mar 18;25(6):1379. doi: 10.3390/molecules25061379.
In this study, we isolated from the aerial parts of Kunth (i) a new neoclerodane, denominated "bacchofertone"; (ii) four known terpenes: schensianol A, bacchofertin, kingidiol and oleanolic acid; and (iii) two flavonoids: cirsimaritin and hispidulin. All structures were identified by an exhaustive analysis of nuclear magnetic resonance (NMR) and mass spectroscopy (MS). Extracts from aerial parts were screened for anti-inflammatory activity in the mice ear edema model of 12--tetradecanoylforbol-13-acetate mice. Dichloromethane extract (BcD) exhibited 78.5 ± 0.72% inhibition of edema, followed by the BcD2 and BcD3 fractions of 71.4% and 82.9% respectively, at a dose of 1 mg/ear. Kingidiol and cirsimaritin were the most potent compounds identified, with a median effective dose of 0.12 and 0.16 mg/ear, respectively. A histological analysis showed that the topical application of TPA promoted intense cell infiltration, and this inflammatory parameter was reduced with the topical application of isolated compounds.
在这项研究中，我们从 Kunth 的地上部分分离出了（i）一种新的新克氏烷，命名为“bacchofertone”；（ii）四种已知的萜类化合物：schensianol A、bacchofertin、kingidiol 和齐墩果酸；和（iii）两种黄酮类化合物：芹菜素和金缕梅素。所有结构均通过核磁共振（NMR）和质谱（MS）的全面分析确定。从地上部分提取的物质在 12--十四烷酰佛波醇-13-乙酸酯诱导的小鼠耳肿胀模型中筛选抗炎活性。二氯甲烷提取物（BcD）在 1 mg/耳剂量下，对水肿的抑制率为 78.5±0.72%，其次是 BcD2 和 BcD3 部分，分别为 71.4%和 82.9%。kingidiol 和芹菜素是鉴定出的最有效化合物，其半数有效剂量分别为 0.12 和 0.16 mg/耳。组织学分析表明，TPA 的局部应用促进了强烈的细胞浸润，而这种炎症参数随着分离化合物的局部应用而降低。
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