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熊蒜添加对波兰红牛和波兰荷斯坦弗里斯兰牛牛奶软干酪化学成分、微生物质量、抗氧化能力和蛋白水解度的影响。

Effect of Bear Garlic Addition on the Chemical Composition, Microbiological Quality, Antioxidant Capacity, and Degree of Proteolysis in Soft Rennet Cheeses Produced from Milk of Polish Red and Polish Holstein-Friesian Cows.

机构信息

Department of Animal Product Technology, University of Agriculture in Krakow, Balicka 122, 30-149 Krakow, Poland.

Department of Food Hygiene and Safety, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia.

出版信息

Molecules. 2022 Dec 15;27(24):8930. doi: 10.3390/molecules27248930.

Abstract

This study aimed to assess the effect of milk source and bear garlic addition on the selected properties of soft rennet cheese. Cheeses were produced from cow milk derived from two sources: Polish Red cows (PR) and Polish Holstein-Friesian cows (PHF) with a 0.5% (/) addition of bear garlic ( L.) dried leaves. Chemical composition and fatty acid profiles (GC) were determined in fresh cheeses. Fresh and stored for two weeks cheeses were subjected to microbiological studies, i.e., total aerobic bacteria count (TABC); count of sp., yeast and molds; coliforms; analysis of the proteolysis extension by means of -phthaldialdehyde (OPA) assay and free amino acids content (HPLC); antioxidant capacity as 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and ferric reducing antioxidant power (FRAP); as well as pH and water activity. Cheeses with bear garlic herbs were more prone to proteolysis but this was not accompanied by any effect on the microbial counts, water activity or pH. Cheeses produced from PR milk contained less monounsaturated fatty acids (MUFA) but were richer in n-3 PUFA and had a lower n-6/n-3 FA ratio than cheeses from PHF milk. Bear garlic addition increased DPPH anti-radical power but had less of an effect on the FRAP values.

摘要

本研究旨在评估奶源和熊蒜添加对软凝乳奶酪某些特性的影响。奶酪由来自两个来源的牛奶制成:波兰红牛(PR)和波兰荷斯坦奶牛(PHF),添加 0.5%(/)熊蒜( L.)干叶。新鲜奶酪中测定了化学成分和脂肪酸谱(GC)。新鲜奶酪和储存两周的奶酪进行了微生物学研究,即总需氧细菌计数(TABC);计数 sp.、酵母和霉菌;大肠菌群;通过 -phthaldialdehyde(OPA)测定法和游离氨基酸含量(HPLC)分析蛋白水解延伸;抗氧化能力作为 2,2-二苯基-1-苦基肼(DPPH)自由基清除活性和铁还原抗氧化能力(FRAP);以及 pH 值和水活度。添加熊蒜草药的奶酪更容易发生蛋白水解,但这并未对微生物计数、水活度或 pH 值产生任何影响。PR 牛奶生产的奶酪含有较少的单不饱和脂肪酸(MUFA),但富含 n-3PUFA,并且 n-6/n-3 FA 比 PHF 牛奶生产的奶酪低。熊蒜的添加增加了 DPPH 抗自由基能力,但对 FRAP 值的影响较小。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7ec9/9787386/f0efc55c38c7/molecules-27-08930-g001.jpg

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