Animal Technology Research Center, Agriculture Technology Research Institute, Miaoli 35053, Taiwan.
Department of Pediatrics, Taipei Hospital, Ministry of Health and Welfare, Taipei 11267, Taiwan.
Nutrients. 2022 Dec 7;14(24):5219. doi: 10.3390/nu14245219.
Global air pollution and diesel exhaust particles (DEPs) generated by intratracheal instillation aggravate asthma. In this study, we evaluated the effect of probiotics via tracheal- or oral-route administration on allergies or asthma. We continuously perfused rats daily, using the oral and tracheal routes, with approximately 10-10 CFU probiotics, for 4 weeks. During this period, we used OVA-sensitized rats to build the asthma models. We orally or intratracheally administered 33 (LP33) to the rats, which reduced the number of total inflammatory cells, lymphocytes, and eosinophils in the bronchoalveolar-lavage fluid, the IgE concentration, and the cytokine levels of TH2 cells, but we found no significant difference in the cytokine levels of TH1 cells. LP33 can be used to prevent asthmatic allergic reactions induced by aerosol particles. Nevertheless, the dosage form or use of LP33 needs to be adjusted to reduce the irritation of lung tissues, which may produce lesions of the trachea. We observed that DEP dosage can alleviate emphysema, and that LP33 has a substantial effect on improving or slowing allergic asthma.
全球空气污染和通过气管内滴注产生的柴油机废气颗粒（DEPs）可加重哮喘。在这项研究中，我们评估了通过气管或口服途径给予益生菌对过敏或哮喘的影响。我们连续 4 周每天通过口腔和气管途径对大鼠进行约 10-10 CFU 的益生菌连续灌注。在此期间，我们使用 OVA 致敏大鼠建立哮喘模型。我们对大鼠进行了口服或气管内 LP33 给药，这降低了支气管肺泡灌洗液中的总炎性细胞、淋巴细胞和嗜酸性粒细胞数量、IgE 浓度和 TH2 细胞的细胞因子水平，但我们发现 TH1 细胞的细胞因子水平没有显著差异。LP33 可用于预防由气溶胶颗粒引起的哮喘过敏反应。然而，LP33 的剂型或使用需要进行调整，以减少对肺组织的刺激，这可能会导致气管损伤。我们观察到 DEP 剂量可以减轻肺气肿，并且 LP33 对改善或减缓过敏性哮喘有显著效果。
