Chen Xin, Wang Chao, Hao Min, Zhao Hang, Xia He, Yu Liyang, Li Dong, Qiu Jichuan, Li Haijun, Han Lin, Sang Yuanhua
State Key Laboratory of Crystal Materials, Shandong University, Jinan 250100, China.
Institute of Marine Science and Technology, Shandong University, Tsingdao 266237, China.
Pharmaceutics. 2022 Dec 6;14(12):2730. doi: 10.3390/pharmaceutics14122730.
Silicon-derived biomaterials are conducive to regulating the fate of osteo-related stem cells, while their effects on the osteogenic differentiation of human adipose-derived stem cells (hADSCs) remain inconclusive. Mesoporous silica (mSiO) is synthesized in a facile route that exhibited the capability of promoting osteogenic differentiation of hADSCs. The metabolism of SiO in cells is proposed according to the colocalization fluorescence analysis between lysosomes and nanoparticles. The released silicon elements promote osteogenic differentiation. The detection of secretory proteins through numerous parallel experiments performed via a microfluidic chip confirms the positive effect of SiO on the osteogenic differentiation of hADSCs. Moreover, constructed with superparamagnetic iron oxide (FeO), the magnetic nanoparticles (MNPs) of FeO@mSiO endow the cells with magnetic resonance imaging (MRI) properties. The MNP-regulated osteogenic differentiation of autologous adipose-derived stem cells provides considerable clinical application prospects for stem cell therapy of bone tissue repair with an effective reduction in immune rejection.
硅基生物材料有利于调节骨相关干细胞的命运，但其对人脂肪来源干细胞（hADSCs）成骨分化的影响仍尚无定论。介孔二氧化硅（mSiO）通过简便的方法合成，具有促进hADSCs成骨分化的能力。根据溶酶体与纳米颗粒之间的共定位荧光分析，提出了SiO在细胞中的代谢情况。释放的硅元素促进成骨分化。通过微流控芯片进行的大量平行实验对分泌蛋白的检测证实了SiO对hADSCs成骨分化的积极作用。此外，FeO@mSiO磁性纳米颗粒（MNPs）由超顺磁性氧化铁（FeO）构建而成，赋予细胞磁共振成像（MRI）特性。MNP调节自体脂肪来源干细胞的成骨分化，为骨组织修复的干细胞治疗提供了可观的临床应用前景，同时有效降低免疫排斥反应。
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