Roopavath Uday Kiran, Soni Raghav, Mahanta Urbashi, Deshpande Atul Suresh, Rath Subha Narayan
Regenerative Medicine and Stem Cell (RMS) Lab, Department of Biomedical Engineering, Indian Institute of Technology Hyderabad (IITH) Kandi (V), Sangareddy (M), Medak-502285 Telangana India
Department of Material Science and Metallurgical Engineering, Indian Institute of Technology Hyderabad Kandi, Medak-502285 Telangana India.
RSC Adv. 2019 Jul 31;9(41):23832-23842. doi: 10.1039/c9ra03641e. eCollection 2019 Jul 29.
Sodium alginate and gelatin are biocompatible & biodegradable natural polymer hydrogels, which are widely investigated for application in tissue engineering using 3D printing and 3D bioprinting fabrication techniques. The major challenge of using hydrogels for tissue fabrication is their lack of regeneration ability, uncontrolled swelling, degradation and inability to hold 3D structure on their own. Free hydroxyl groups on the surface of SiO nanoparticles have the ability to chemically interact with alginate-gelatin polymer network, which can be explored to achieve the above parameters. Hence validating the incorporation of SiO nanoparticles in a 3D printable hydrogel polymer network, according to the patient's critical defects has immense scope in bone tissue engineering. In this study, SiO nanoparticles are loaded into alginate-gelatin composite hydrogels and chemically crosslinked with CaCl solution. The effect of SiO nanoparticles on the viscosity, swelling, degradation, compressive modulus (MPa), biocompatibility and osteogenic ability were evaluated on lyophilized scaffolds and found to be desirable for bone tissue engineering. A complex irregular patient-specific virtual defect was created and the 3D printing process to fabricate such structures was evaluated. The 3D printing of SiO nanoparticle hydrogel composite ink to fabricate a bone graft using a patient-specific virtual defect was successfully validated. Hence this type of hydrogel composite ink has huge potential and scope for its application in tissue engineering and nanomedicine.
海藻酸钠和明胶是具有生物相容性和可生物降解性的天然聚合物水凝胶，它们在使用3D打印和3D生物打印制造技术的组织工程应用中得到了广泛研究。将水凝胶用于组织制造的主要挑战在于其缺乏再生能力、肿胀不受控制、降解以及无法自行保持3D结构。SiO纳米颗粒表面的游离羟基能够与海藻酸钠-明胶聚合物网络发生化学相互作用，可利用这一点来实现上述参数。因此，根据患者的关键缺损情况，验证SiO纳米颗粒在可3D打印水凝胶聚合物网络中的掺入情况，在骨组织工程中具有广阔的应用前景。在本研究中，将SiO纳米颗粒负载到海藻酸钠-明胶复合水凝胶中，并与CaCl溶液进行化学交联。在冻干支架上评估了SiO纳米颗粒对粘度、肿胀、降解、压缩模量（MPa）、生物相容性和成骨能力的影响，发现其对骨组织工程具有理想的效果。创建了一个复杂的不规则患者特异性虚拟缺损，并评估了制造此类结构的3D打印过程。成功验证了使用患者特异性虚拟缺损通过3D打印SiO纳米颗粒水凝胶复合墨水来制造骨移植体。因此，这种类型的水凝胶复合墨水在组织工程和纳米医学中的应用具有巨大的潜力和前景。
