Xia He, Dong Lun, Hao Min, Wei Yuan, Duan Jiazhi, Chen Xin, Yu Liyang, Li Haijun, Sang Yuanhua, Liu Hong
State Key Laboratory of Crystal Materials, Shandong University, Jinan, China.
Department of Breast Surgery, Qilu Hospital, Shandong University, Jinan, China.
Front Chem. 2021 Nov 19;9:798299. doi: 10.3389/fchem.2021.798299. eCollection 2021.
Cancellous bone plays an indispensable role in the skeletal system due to its various functions and high porosity. In this work, chitosan and hydroxyapatite nanowires (CS@HAP NWs) hybrid nanostructured scaffolds with suitable mechanical properties, high porosity and a fine porous structure were prepared to simulate the 3-dimensional structure of cancellous bone. The 3D-hybrid scaffolds promote cell adhesion and the migration of human adipose-derived stem cells (hADSCs) inside the scaffolds. The cavities in the scaffolds provide space for the hADSCs proliferation and differentiation. Moreover, the various contents of HAP and the induced mechanical property changes regulate the differentiation of hADSCs toward osteoblasts. Overall, cellular fate regulation of hADSCs via rationally engineered HAP-based hybrid scaffolds is a facile and effective approach for bone tissue engineering.
由于其多种功能和高孔隙率，松质骨在骨骼系统中发挥着不可或缺的作用。在这项工作中，制备了具有合适机械性能、高孔隙率和精细多孔结构的壳聚糖和羟基磷灰石纳米线（CS@HAP NWs）混合纳米结构支架，以模拟松质骨的三维结构。这种三维混合支架促进人脂肪来源干细胞（hADSCs）在支架内的黏附和迁移。支架中的空隙为hADSCs的增殖和分化提供了空间。此外，HAP的不同含量以及由此引起的机械性能变化调节hADSCs向成骨细胞的分化。总体而言，通过合理设计的基于HAP的混合支架对hADSCs进行细胞命运调控是一种简便有效的骨组织工程方法。
