Ageyeva Maria, Veselov Alexander, Vodeneev Vladimir, Brilkina Anna
Department of Biochemistry and Biotechnology, National Research Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Avenue, Nizhny Novgorod 603950, Russia.
Department of Biophysics, National Research Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Avenue, Nizhny Novgorod 603950, Russia.
Plants (Basel). 2022 Dec 15;11(24):3532. doi: 10.3390/plants11243532.
Soil salinity negatively affects the growth, development and yield of plants. Acidification of the cytosol in cells of glycophytes was reported under salinity, while various types of plant cells can have a specific reaction under the same conditions. Transgenic plants expressing the pH sensor Pt-GFP in the cytosol were used in this work for determination of morphometric changes and cytosolic pH changes in the superficial cells of roots under chronic salinity in vitro. We did not find changes in the length of the root cap cells, while there was a decrease in the length of the differentiation zone under 50, 75 mM NaCl and the size of the epidermal cells of the differentiation zone under 75 mM NaCl. The most significant changes of cytosolic pH to chronic salinity was noted in columella (decrease by 1 pH unit at 75 mM NaCl) and epidermal cells of the differentiation zone (decrease by 0.6 and 0.4 pH units at 50 and 75 mM NaCl, respectively). In developed lateral root cap cells, acidification of cytosol by 0.4 units occurred only under 75 mM NaCl in the medium. In poorly differentiated lateral cells of the root cap, there were no changes in pH under chronic salinity.
土壤盐度对植物的生长、发育和产量产生负面影响。据报道，在盐度条件下，甜土植物细胞的胞质溶胶会发生酸化，而在相同条件下，不同类型的植物细胞可能会有特定的反应。在这项工作中，使用在胞质溶胶中表达pH传感器Pt-GFP的转基因植物，来测定体外长期盐度处理下根表面细胞的形态计量学变化和胞质pH变化。我们没有发现根冠细胞长度的变化，而在50、75 mM NaCl处理下，分化区长度减小，在75 mM NaCl处理下，分化区表皮细胞大小减小。在柱细胞（在75 mM NaCl时降低1个pH单位）和分化区表皮细胞（在50和75 mM NaCl时分别降低0.6和0.4个pH单位）中，观察到胞质pH对长期盐度的最显著变化。在发育良好的侧根冠细胞中，仅在培养基中75 mM NaCl处理下，胞质溶胶酸化0.4个单位。在根冠分化较差的侧细胞中，长期盐度处理下pH没有变化。
