Sprague D B, Gadian D G, Williams S R, Proctor E, Goode A W
Department of Physics in Relation to Surgery, Royal College of Surgeons of England, London.
J R Soc Med. 1987 Aug;80(8):495-8. doi: 10.1177/014107688708000813.
Hind limb skeletal muscle was studied in vivo in a rat trauma model using nuclear magnetic resonance (NMR) spectroscopy. The model used was a 25% body surface area, full-thickness burn administered under anaesthesia. Two groups of six rats were studied. Weight loss was observed in the experimental group whilst the control group continued to gain weight. Concentration ratios involving intramyocellular phosphocreatine (PCr), creatine (Cr), adenosine triphosphate (ATP), inorganic phosphate (Pi), anserine (Ans) and taurine (Tau) were measured. No change in the ratios of PCr/Pi, PCr/ATP, Ans/PCr + Cr and Tau/PCr + Cr were seen between the two groups. Intracellular pH was the same in the two groups. NMR spectroscopy in vivo gives values of Pi and PCr that differ from those obtained by conventional techniques. NMR values are probably more accurate as no degradation occurs during measurement, the measurements being repeatable and noninvasive.
在大鼠创伤模型中，利用核磁共振（NMR）光谱技术对后肢骨骼肌进行了体内研究。所采用的模型是在麻醉状态下造成25%体表面积的全层烧伤。对两组各6只大鼠进行了研究。实验组观察到体重减轻，而对照组体重持续增加。测量了涉及肌细胞内磷酸肌酸（PCr）、肌酸（Cr）、三磷酸腺苷（ATP）、无机磷酸盐（Pi）、鹅肌肽（Ans）和牛磺酸（Tau）的浓度比。两组之间PCr/Pi、PCr/ATP、Ans/PCr + Cr和Tau/PCr + Cr的比值没有变化。两组的细胞内pH值相同。体内NMR光谱得出的Pi和PCr值与传统技术获得的值不同。NMR值可能更准确，因为在测量过程中不会发生降解，测量具有可重复性且是非侵入性的。
