Ohmori H, Ienaga M, Yamamoto I
Department of Immunochemistry, Faculty of Pharmaceutical Sciences, Okayama University, Japan.
Jpn J Pharmacol. 1987 Jun;44(2):225-7. doi: 10.1254/jjp.44.225.
We have devised a new culture medium that is made of RPMI-1640 medium, 500 micrograms/ml beta-cyclodextrin (beta-CD) and 1% fetal calf serum (FCS) (beta-CD medium). Murine lymphocytes stimulated with sheep erythrocytes in vitro developed antibody-forming cells in beta-CD medium as efficiently as in 10% FCS-containing RPMI-1640 medium (10F medium). Immunostimulating agents (SA96 and levamisole) and immunosuppressive agents (hydrocortisone and D-penicillamine) showed similar immunomodulating effects on the antibody responses in both media. In addition, the enhancing effect of levamisole varied drastically depending on the lot of FCS used in 10F medium, but a clear enhancement was always observed in beta-CD medium containing any lot of FCS tested.
