Ohmori H
Department of Biotechnology, Faculty of Engineering, Okayama University, Japan.
J Immunol Methods. 1988 Sep 13;112(2):227-33. doi: 10.1016/0022-1759(88)90362-6.
A serum-free medium has been developed which is able to support primary antibody responses by cultured murine lymphocytes. This medium is based on RPMI 1640 that is supplemented with beta-cyclodextrin, insulin, transferrin, albumin, low density lipoprotein, putrescine and L-alanine as substitutes for fetal calf serum. Omission of anyone of these components resulted in a marked decrease of antibody responses. By employing the serum-free culture conditions, it was clearly demonstrated that polyamines such as putrescine, spermidine and spermine had positive effects on the development of antibody-forming cells. This serum-free medium supported the antibody response to sheep erythrocytes, trinitrophenyl (TNP)-Ficoll or TNP-lipopolysaccharide as efficiently as 10% fetal calf serum-containing medium. In addition, murine lymphocytes proliferated in response to an antigen or a mitogen equally well in these two types of medium.
已开发出一种无血清培养基，它能够支持培养的小鼠淋巴细胞产生初次抗体反应。该培养基以RPMI 1640为基础，添加了β-环糊精、胰岛素、转铁蛋白、白蛋白、低密度脂蛋白、腐胺和L-丙氨酸作为胎牛血清的替代品。省略这些成分中的任何一种都会导致抗体反应显著下降。通过采用无血清培养条件，清楚地证明了腐胺、亚精胺和精胺等多胺对抗体形成细胞的发育有积极作用。这种无血清培养基支持对绵羊红细胞、三硝基苯基（TNP）-Ficoll或TNP-脂多糖的抗体反应，其效率与含10%胎牛血清的培养基相同。此外，在这两种培养基中，小鼠淋巴细胞对抗原或有丝分裂原的增殖反应同样良好。
