将lacI基因克隆到一个ColE1质粒中。
Cloning of the lacI gene into A ColE1 plasmid.
作者信息
Hare D L, Sadler J R
出版信息
Gene. 1978 Jul;3(4):269-78. doi: 10.1016/0378-1119(78)90037-9.
The binding of lac repressor to lac operator was utilized to isolate an EcoRI fragment of lambda h80dlac (i+ as well as iq) that contains the lacI gene and lac promoter-operator regions. Ligation of this fragment into EcoRI cleaved pMB9 yielded chimeric DNA molecules of mol. w.t. 9.6 . 10(-6) and 1.5 . 10(7) daltons. Transformed strains containing the plasmids were analyzed for repressor production in vivo and in vitro. Repressor production in one plasmid strain is 7-fold greater than that in heat-inducible lambda h80dlac(iq)lysogens.
利用乳糖阻遏物与乳糖操纵基因的结合来分离λh80dlac（i⁺以及iᴵˡ）的一个EcoRI片段，该片段包含lacI基因和乳糖启动子-操纵基因区域。将此片段连接到用EcoRI切割的pMB9中，产生了分子量分别为9.6×10⁻⁶和1.5×10⁷道尔顿的嵌合DNA分子。对含有这些质粒的转化菌株进行了体内和体外阻遏物产生情况的分析。一个质粒菌株中的阻遏物产量比热诱导型λh80dlac（iᴵˡ）溶源菌中的产量高7倍。
Zotero 插件