Ellenbecker Mary, Voronina Ekaterina
University of Montana.
MicroPubl Biol. 2022 Dec 6;2022. doi: 10.17912/micropub.biology.000700. eCollection 2022.
Dynein light chain (DLC-1) is a light chain component of the dynein motor complex, it functions as an allosteric regulator of multi-subunit protein complexes and promotes P granule integrity in the embryo. P granules are RNA-protein complexes located in the germline that are important for RNA regulation and fertility. To further study the role of DLC-1 during embryogenesis we performed quantitative tandem mass tag mass spectrometry on embryos after knock down. The amount of core P granule components and nucleoporin proteins did not change after These results show that DLC-1 does not help regulate P granule protein levels and support the model that DLC-1 facilitates phase separation of P granule components .
动力蛋白轻链（DLC-1）是动力蛋白运动复合体的轻链成分，它作为多亚基蛋白复合体的变构调节剂，促进胚胎中P小体的完整性。P小体是位于生殖系中的RNA-蛋白质复合体，对RNA调控和生育能力很重要。为了进一步研究DLC-1在胚胎发生过程中的作用，我们对敲低后的胚胎进行了定量串联质量标签质谱分析。核心P小体成分和核孔蛋白的量在敲低后没有变化。这些结果表明，DLC-1无助于调节P小体蛋白水平，并支持DLC-1促进P小体成分相分离的模型。
