Khan Naimat Ullah, Qazi Neelum Gul, Khan Arif-Ullah, Ali Fawad, Hassan Syed Shams Ul, Bungau Simona
Riphah Institute of Pharmaceutical Sciences, Riphah International University, Islamabad44000, Pakistan.
Department of Pharmacy, Kohat University of Science and Technology,Kohat2600, Pakistan.
ACS Omega. 2022 Dec 8;7(50):46358-46370. doi: 10.1021/acsomega.2c04977. eCollection 2022 Dec 20.
Diabetes mellitus (DM) is a complex and multiple group of disorders, and understanding the molecular mechanisms is a key role in identifying various markers involved in the diagnosis of the disease. Brucine is derived from the seeds of L. (Loganiaceae), which has been used in traditional medicine to cure a variety of ailments, such as chronic rheumatism, nervous system diseases, dyspepsia, gonorrhea, anemia, and bronchitis, and has analgesic, anti-inflammatory, anti-oxidant, anti-snake venom, and anti-diabetic properties. The anti-diabetic potential of brucine was studied utilizing , , , and molecular methods, including streptozotocin-induced diabetic rat models, α-glucosidase and α-amylase inhibitory assays, and via Auto-DocVina software. Brucine exhibits binding affinities of -5.0 to -10.1 Kcal/mol against chosen protein targets, according to an investigation. studies revealed that brucine inhibited the enzymes α-amylase and α-glucosidase, and brucine (20 mg/kg) reduced blood glucose levels, oral glucose tolerance overload, body weight, glycosylated hemoglobin levels, total cholesterol, triglycerides, low-density lipoprotein, alanine transaminase, aspartate aminotransferase, total bilirubin, and alkaline phosphatase and elevated high-density lipoprotein levels in studies The brucine binding energy against certain protein targets ranges from -5.0 to -10.1 Kcal/mol. It has anti-diabetic, anti-hyperlipidemic, hepatoprotective, anti-oxidant, and anti-inflammatory properties, which are mediated via inhibition of α-glucosidase and α-amylase.
糖尿病（DM）是一组复杂的多种疾病，了解其分子机制对于识别参与该疾病诊断的各种标志物至关重要。马钱子碱源自马钱科植物的种子，在传统医学中被用于治疗多种疾病，如慢性风湿病、神经系统疾病、消化不良、淋病、贫血和支气管炎，具有镇痛、抗炎、抗氧化、抗蛇毒和抗糖尿病特性。利用链脲佐菌素诱导的糖尿病大鼠模型、α-葡萄糖苷酶和α-淀粉酶抑制试验以及通过Auto-DocVina软件等体外、体内和分子方法研究了马钱子碱的抗糖尿病潜力。根据一项分子对接研究，马钱子碱对选定的蛋白质靶点表现出-5.0至-10.1千卡/摩尔的结合亲和力。体外研究表明，马钱子碱抑制了α-淀粉酶和α-葡萄糖苷酶，体内研究表明，马钱子碱（20毫克/千克）降低了血糖水平、口服葡萄糖耐量负荷、体重、糖化血红蛋白水平、总胆固醇、甘油三酯、低密度脂蛋白、丙氨酸转氨酶、天冬氨酸转氨酶、总胆红素和碱性磷酸酶，并提高了高密度脂蛋白水平。马钱子碱对某些蛋白质靶点的结合能范围为-5.0至-10.1千卡/摩尔。它具有抗糖尿病、抗高血脂、保肝、抗氧化和抗炎特性，这些特性是通过抑制α-葡萄糖苷酶和α-淀粉酶介导的。
