bta-miR-2904 通过靶向病毒感染诱导的自噬来抑制牛病毒性腹泻病毒的复制，该自噬通过 ATG13 进行。
bta-miR-2904 inhibits bovine viral diarrhea virus replication by targeting viral-infection-induced autophagy via ATG13.
机构信息
College of Animal Science and Technology, Shihezi University, 832003, Shihezi, Xinjiang, China.
College of Life Science, Shihezi University, 832003, Shihezi, Xinjiang, China.
出版信息
Arch Virol. 2022 Dec 28;168(1):11. doi: 10.1007/s00705-022-05630-4.
MicroRNAs (miRNAs) are endogenous small and noncoding RNA molecules (18-25 nt) that can regulate expression of their target genes post-transcriptionally. Previously, using high-throughput sequencing data obtained on a Solexa platform, we found that Bos taurus bta-miR-2904 (miR-2904) was significantly upregulated in Madin-Darby bovine kidney (MDBK) cells infected with bovine viral diarrhea virus (BVDV) strain NADL at 2, 6, and 18 h postinfection (hpi) compared to uninfected MDBK cells. Moreover, miR-2904 overexpression significantly reduced BVDV replication. However, the mechanism by which miR-2904 inhibits viral replication remains unclear. In this study, we used electron microscopy, laser confocal microscopy, dual-luciferase reporter analysis, real-time PCR, and Western blot assays to investigate the effect of the miR-2904 expression on BVDV NADL replication and virus-infection-induced autophagy. The results indicate that miR-2904 inhibits autophagy of MDBK cells by targeting autophagy-related gene 13 (ATG13), and overexpression of miR-2904 inhibited the replication of BVDV NADL.
微小 RNA（miRNAs）是内源性的小非编码 RNA 分子（18-25nt），可以在后转录水平上调节其靶基因的表达。此前，我们使用 Solexa 平台获得的高通量测序数据发现，与未感染的 MDBK 细胞相比，感染牛病毒性腹泻病毒（BVDV）NADL 株的 MDBK 细胞在感染后 2、6 和 18 小时（hpi）时，Bos taurus bta-miR-2904（miR-2904）的表达显著上调。此外，miR-2904 的过表达显著降低了 BVDV 的复制。然而，miR-2904 抑制病毒复制的机制尚不清楚。在本研究中，我们使用电子显微镜、激光共聚焦显微镜、双荧光素酶报告分析、实时 PCR 和 Western blot 分析来研究 miR-2904 对 BVDV NADL 复制和病毒感染诱导的自噬的影响。结果表明，miR-2904 通过靶向自噬相关基因 13（ATG13）抑制 MDBK 细胞的自噬，miR-2904 的过表达抑制了 BVDV NADL 的复制。
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