College of Animal Science and Technology, Shihezi University, 832003, Shihezi, Xinjiang, China.
College of Life Science, Shihezi University, 832003, Shihezi, Xinjiang, China.
Arch Virol. 2022 Dec 28;168(1):11. doi: 10.1007/s00705-022-05630-4.
MicroRNAs (miRNAs) are endogenous small and noncoding RNA molecules (18-25 nt) that can regulate expression of their target genes post-transcriptionally. Previously, using high-throughput sequencing data obtained on a Solexa platform, we found that Bos taurus bta-miR-2904 (miR-2904) was significantly upregulated in Madin-Darby bovine kidney (MDBK) cells infected with bovine viral diarrhea virus (BVDV) strain NADL at 2, 6, and 18 h postinfection (hpi) compared to uninfected MDBK cells. Moreover, miR-2904 overexpression significantly reduced BVDV replication. However, the mechanism by which miR-2904 inhibits viral replication remains unclear. In this study, we used electron microscopy, laser confocal microscopy, dual-luciferase reporter analysis, real-time PCR, and Western blot assays to investigate the effect of the miR-2904 expression on BVDV NADL replication and virus-infection-induced autophagy. The results indicate that miR-2904 inhibits autophagy of MDBK cells by targeting autophagy-related gene 13 (ATG13), and overexpression of miR-2904 inhibited the replication of BVDV NADL.
微小 RNA（miRNAs）是内源性的小非编码 RNA 分子（18-25nt），可以在后转录水平上调节其靶基因的表达。此前，我们使用 Solexa 平台获得的高通量测序数据发现，与未感染的 MDBK 细胞相比，感染牛病毒性腹泻病毒（BVDV）NADL 株的 MDBK 细胞在感染后 2、6 和 18 小时（hpi）时，Bos taurus bta-miR-2904（miR-2904）的表达显著上调。此外，miR-2904 的过表达显著降低了 BVDV 的复制。然而，miR-2904 抑制病毒复制的机制尚不清楚。在本研究中，我们使用电子显微镜、激光共聚焦显微镜、双荧光素酶报告分析、实时 PCR 和 Western blot 分析来研究 miR-2904 对 BVDV NADL 复制和病毒感染诱导的自噬的影响。结果表明，miR-2904 通过靶向自噬相关基因 13（ATG13）抑制 MDBK 细胞的自噬，miR-2904 的过表达抑制了 BVDV NADL 的复制。
