Kadiri Mahendra, Sevugapperumal Nakkeeran, Nallusamy Saranya, Ragunathan Janani, Ganesan Malathi Varagur, Alfarraj Saleh, Ansari Mohammad Javed, Sayyed R Z, Lim Hooi Ren, Show Pau Loke
Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore, 641003, India.
Department of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, 641003, India.
Microbiol Res. 2023 Mar;268:127277. doi: 10.1016/j.micres.2022.127277. Epub 2022 Dec 13.
Management of late blight of potato incited by Phytophthora infestans remains a major challenge. Coevolution of pathogen with resistant strains and the rise of fungicide resistance have made it more challenging to prevent the spread of P. infestans. Here, the anti-oomycete potential of Bacillus velezensis VB7 against P. infestans through pan-genome analysis and molecular docking were explored. The Biocontrol potential of VB7 against P. infestans was assessed using a confrontational assay. The biomolecules from the inhibition zone were identified and subjected to in silico analysis against P. infestans target proteins. Nucleotide sequences for 54 B. velezensis strains from different geographical locations were used for pan-genome analysis. The confrontational assay revealed the anti-oomycetes potential of VB7 against P. infestans. Molecular docking confirmed that the penicillamine disulfide had the maximum binding energy with eight effector proteins of P. infestans. Besides, scanning electron microscopic observations of P. infestans interaction with VB7 revealed structural changes in hypha and sporangia. Pan-genome analysis between 54 strains of B. velezensis confirmed that the core genome had 2226 genes, and it has an open pan-genome. The present study confirmed the anti-oomycete potential of B. velezensis VB7 against P. infestans and paved the way to explore the genetic potential of VB7.
由致病疫霉引起的马铃薯晚疫病的管理仍然是一项重大挑战。病原体与抗性菌株的共同进化以及杀菌剂抗性的增加使得预防致病疫霉的传播更具挑战性。在此，通过泛基因组分析和分子对接探索了贝莱斯芽孢杆菌VB7对致病疫霉的抗卵菌潜力。使用对峙试验评估了VB7对致病疫霉的生物防治潜力。鉴定了来自抑制区的生物分子，并对致病疫霉靶蛋白进行了计算机分析。使用来自不同地理位置的54株贝莱斯芽孢杆菌菌株的核苷酸序列进行泛基因组分析。对峙试验揭示了VB7对致病疫霉的抗卵菌潜力。分子对接证实青霉胺二硫化物与致病疫霉的八种效应蛋白具有最大结合能。此外，对致病疫霉与VB7相互作用的扫描电子显微镜观察揭示了菌丝和孢子囊的结构变化。对54株贝莱斯芽孢杆菌进行的泛基因组分析证实，核心基因组有2226个基因，并且具有开放的泛基因组。本研究证实了贝莱斯芽孢杆菌VB7对致病疫霉的抗卵菌潜力，并为探索VB7的遗传潜力铺平了道路。
