Mis Baran, Karaca Kemal, Eltem Rengin
Department of Bioengineering, Graduate School of Natural and Applied Sciences, Ege University, Izmir, Turkey.
Department of Bioengineering, Faculty of Engineering, Ege University, Izmir, Turkey.
J Basic Microbiol. 2024 Dec;64(12):e2400422. doi: 10.1002/jobm.202400422. Epub 2024 Oct 3.
During the cultivation of button mushrooms, the green mold epidemic, which causes a decrease in productivity, is a very important problem. The environmental harm of chemicals used in the control of such epidemics and the demand of consumers for organic products without chemicals have brought environmentally friendly biological control to the fore. Biological control can be achieved by the use of antagonistic microorganisms and their metabolites. In this study, the effectiveness of Bacillus spp. and Pseudomonas spp. for the biological control of the aggressive biotypes of the green mold disease agent Trichoderma aggressivum strains was examined in vitro. For this purpose, the antifungal effects of Bacillus spp. and Pseudomonas spp. against T. aggressivum strains were examined by in vitro dual culture test. Afterward, the antifungal activity of Bacillus spp. metabolites was assessed further using the agar well diffusion method. Then, it was determined whether the bacterial strains showing antifungal activity showed antagonistic activity against A. bisporus. Although none of the Pseudomonas spp. showed antifungal activity against T. aggressivum strains, most of the Bacillus spp. were found to have high activity. It has been concluded that Bacillus sp. Ö-4-82 may be potential biological control agent for button mushroom cultivation.
在双孢蘑菇栽培过程中，导致产量下降的绿霉病是一个非常重要的问题。用于控制此类病害的化学物质对环境造成的危害以及消费者对无化学物质有机产品的需求，使得环境友好型生物防治备受关注。生物防治可通过使用拮抗微生物及其代谢产物来实现。在本研究中，体外检测了芽孢杆菌属和假单胞菌属对绿霉病病原菌侵略性木霉菌株的强致病型进行生物防治的效果。为此，通过体外共培养试验检测了芽孢杆菌属和假单胞菌属对侵略性木霉菌株的抗真菌作用。之后，使用琼脂扩散法进一步评估了芽孢杆菌属代谢产物的抗真菌活性。然后，确定表现出抗真菌活性的细菌菌株是否对双孢蘑菇具有拮抗活性。虽然假单胞菌属均未对侵略性木霉菌株表现出抗真菌活性，但发现大多数芽孢杆菌属具有高活性。得出结论，芽孢杆菌属Ö-4-82可能是双孢蘑菇栽培的潜在生物防治剂。
