King T P, Kochoumian L, Lam T
Rockefeller University, New York, NY 10021.
Mol Immunol. 1987 Aug;24(8):857-64. doi: 10.1016/0161-5890(87)90188-x.
Antigen 5 is a venom protein of hornets, yellowjackets and wasps, and it is an important allergen for insect-sensitive patients. Antigen 5s from these insects are found to have similar amino-terminal sequences for their first 20 residues, and to have similar cyanogen bromide cleavage patterns. A partial sequence homology of antigen 5s and scorpion neurotoxins is observed. Antigen 5s from these insects show varying extents of cross reactivity when tested with mouse polyclonal antibodies by enzyme immunoassay and they show limited or no cross reactivity with mouse monoclonal antibodies. Chemically modified antigen 5s shown greatly decreased binding of antigen 5-specific mouse polyclonal antibodies. The modified antigen 5s include its reduced and carboxymethylated
抗原5是黄蜂、黄胡蜂和胡蜂的一种毒液蛋白，对于昆虫敏感患者来说它是一种重要的过敏原。已发现这些昆虫的抗原5在其前20个残基的氨基末端序列相似，并且具有相似的溴化氰裂解模式。观察到抗原5与蝎神经毒素存在部分序列同源性。当通过酶免疫测定法用小鼠多克隆抗体进行检测时，这些昆虫的抗原5表现出不同程度的交叉反应性，并且它们与小鼠单克隆抗体的交叉反应性有限或无交叉反应性。化学修饰的抗原5显示出与抗原5特异性小鼠多克隆抗体的结合大大减少。修饰后的抗原5包括其还原型和羧甲基化型
