Afsharian Zahra, Salavatifar Maryam, Khosravi Darani Kianoush
Department of Food Science and Technology, National Nutrition and Food Technology Research Institute, Faculty of Nutrition Science and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Aerospace Research Institute, Ministry of Science, Research and Technology, Tehran, Iran.
Heliyon. 2022 Dec 14;8(12):e12307. doi: 10.1016/j.heliyon.2022.e12307. eCollection 2022 Dec.
There are several reports about the effect of gravity removal on some characteristics of microorganisms due to possible change in surface layer thickness and adherence properties. In this study, bioremoval efficiency of ATCC 4356 from water under simulated microgravity conditions was investigated. Furthermore, pretreatment effects (untreated, NaOH, and heat pretreated) of ATCC 4356 on heavy metal removal was evaluated on microgravity, as our previous research showed impact of pretreatment on adherence properties of probiotics to environmental metals. The results showed that ability of for arsenic adsorption enhanced following heat-pretreatment in simulated and normal gravity. Moreover, in both conditions of simulated microgravity and normal gravity NaOH-treated increased the removal of cadmium and lead. In none of the conditions, pretreatment of lactobacillus affects mercury removal. Evaluation of stability of binding of -heavy metal was investigated to check irreversibility of complex formation between microorganisms and metals in simulated gastrointestinal conditions. Data showed release of heavy metals from complex in normal gravity. Obtained results of this research show the favorable potential of simulated microgravity condition to increase bioremoval capacity of for heavy metals.
有几份报告指出,由于表面层厚度和附着特性可能发生变化,去除重力对微生物的某些特性会产生影响。在本研究中,研究了在模拟微重力条件下从水中去除ATCC 4356的生物去除效率。此外,由于我们之前的研究表明预处理对益生菌与环境金属的附着特性有影响,因此在微重力条件下评估了ATCC 4356的预处理效果(未处理、氢氧化钠处理和热处理)对重金属去除的影响。结果表明,在模拟重力和正常重力条件下,热处理后对砷的吸附能力增强。此外,在模拟微重力和正常重力条件下,氢氧化钠处理均增加了镉和铅的去除量。在任何条件下,乳酸菌的预处理均不影响汞的去除。研究了重金属结合稳定性,以检查在模拟胃肠道条件下微生物与金属之间形成络合物的不可逆性。数据显示在正常重力条件下重金属从络合物中释放出来。本研究获得的结果表明,模拟微重力条件在提高对重金属的生物去除能力方面具有良好的潜力。