Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, International Innovation Center for Forest Chemicals and Materials, College of Light Industry and Food, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China.
Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, International Innovation Center for Forest Chemicals and Materials, College of Light Industry and Food, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Apr 5;290:122268. doi: 10.1016/j.saa.2022.122268. Epub 2022 Dec 20.
Hypochlorous acid (HClO) plays an important role in many physiological and pathological activities. In this work, a novel BODIPY-based Near-infrared (NIR) ratiometric fluorescent probe BODIPY-Hyp was designed for the rapid detection of HClO. The probe BODIPY-Hyp was highly selective and sensitive for HClO with a low detection limit of 16.74 nM and short response time of less than 60 s. The probe BODIPY-Hyp in response to HClO exhibited a significant blue-shifted fluorescence emission from 700 nm to 530 nm, and its fluorescence intensity ratio (I/I) increased about 1200 times before and after adding HClO. Moreover, the reaction mechanism of BODIPY-Hyp with HClO was verified by HRMS analysis, H NMR titration and DFT calculations. Furthermore, BODIPY-Hyp was successfully processed into a portable test strip-based device for the detection of HClO. In addition, the probe BODIPY-Hyp could be used in real time to monitor the levels of HClO in living zebrafish larvae. In conclusion, BODIPY-Hyp has great application potential in the life and environmental sciences.
次氯酸（HClO）在许多生理和病理活动中发挥着重要作用。在这项工作中，设计了一种基于 BODIPY 的新型近红外（NIR）比率荧光探针 BODIPY-Hyp，用于快速检测 HClO。探针 BODIPY-Hyp 对 HClO 具有高选择性和灵敏度，检测限低至 16.74 nM，响应时间小于 60 s。探针 BODIPY-Hyp 对 HClO 的响应表现出明显的蓝移荧光发射，从 700 nm 到 530 nm，其荧光强度比（I/I）在加入 HClO 前后增加了约 1200 倍。此外，通过 HRMS 分析、H NMR 滴定和 DFT 计算验证了 BODIPY-Hyp 与 HClO 的反应机制。此外，BODIPY-Hyp 成功加工成基于便携式测试条的设备，用于检测 HClO。此外，该探针 BODIPY-Hyp 可用于实时监测活斑马鱼幼虫中 HClO 的水平。总之，BODIPY-Hyp 在生命和环境科学中有很大的应用潜力。
