Yang Taorui, An Ke, Zhang Shishen, Han Yifeng
Department of Chemistry, Zhejiang Sci-Tech University, Hangzhou 310018, China.
Department of Chemistry, Zhejiang Sci-Tech University, Hangzhou 310018, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Aug 5;296:122655. doi: 10.1016/j.saa.2023.122655. Epub 2023 Mar 21.
Due to the highly significant biological activity of hypochlorous acid, the monitoring of its concentration in vivo has received extensive attention. In this work, a photoinduced electron transfer (PeT) based benzo-bodipy fluorescent probe BBy-T has been developed for the rapid, sensitive, and selective detection of HClO in an aqueous solution. Based on the HClO-specific oxidation reaction, BBy-T exhibited a distinct fluorescence turn-on response to HClO with a remarkable Stokes shift (84 nm), immediate response (less than 20 s), and low detection limit (13.7 nM). In addition, the bioimaging results indicated that the probe BBy-T could be applied to real-time fluorescence imaging of living HeLa cells as well as living zebrafish.
由于次氯酸具有高度显著的生物活性，其体内浓度的监测受到了广泛关注。在这项工作中，基于光致电子转移（PeT）的苯并硼二吡咯荧光探针BBy-T被开发用于在水溶液中快速、灵敏且选择性地检测HClO。基于HClO特异性氧化反应，BBy-T对HClO表现出明显的荧光开启响应，具有显著的斯托克斯位移（84 nm）、即时响应（小于20秒）和低检测限（13.7 nM）。此外，生物成像结果表明，探针BBy-T可应用于活HeLa细胞以及活斑马鱼的实时荧光成像。
