Ma Jiao, Shen Zhan, Peng Rongmei, Li Chendi, Zhao Yinghan, Hu Bohao, Hong Jing
Department of Ophthalmology, Peking University Third Hospital, Beijing, China ; and.
Beijing Key Laboratory of Restoration of Damaged Ocular Nerve, Peking University Third Hospital, Beijing, China .
Cornea. 2023 Feb 1;42(2):211-216. doi: 10.1097/ICO.0000000000003081. Epub 2022 Jul 4.
The local application of antiinflammatory and immunosuppressive agents is an effective method for the treatment of ocular graft-versus-host disease (oGVHD); however, we noticed that some patients with oGVHD did not respond to topical therapy as well as many others. This study aimed to determine whether tear cytokines were associated with therapeutic effects in oGVHD.
Forty patients with chronic oGVHD were enrolled and grouped as responders (n = 24) and nonresponders (n = 16) based on the clinical response to 1 month of topical treatment. Tear samples were collected from each participant before and after treatment, and the tear concentrations of 7 cytokines (IL-2, IL-6, IL-8, IL-10, IL-17A, TNF-α, and ICAM-1) were measured using microsphere-based immunoassay analysis. Differences between pretreatment and posttreatment tear samples were analyzed using the Wilcoxon test.
No significant differences in ophthalmic symptoms or cytokine levels were observed between responders and nonresponders at baseline. After 1 month of topical treatment, ocular surface parameters (including Ocular Surface Disease Index, National Institutes of Health eye score, best-corrected visual acuity, corneal fluorescein staining score, and fluorescein tear film break-up time) were significantly ameliorated in responders, but not in nonresponders. Moreover, none of the cytokines exhibited significant alteration in nonresponders, whereas the tear levels of IL-6 (P = 0.031) and IL-8 (P = 0.037) exhibited significant decreases in responding patients.
Our results revealed that tear IL-6 and IL-8 levels were significantly altered in response to topical oGVHD treatment.
局部应用抗炎和免疫抑制剂是治疗眼部移植物抗宿主病（oGVHD）的有效方法；然而，我们注意到一些oGVHD患者对局部治疗的反应不如其他许多患者。本研究旨在确定泪液细胞因子是否与oGVHD的治疗效果相关。
招募40例慢性oGVHD患者，根据对1个月局部治疗的临床反应分为反应者（n = 24）和无反应者（n = 16）。在治疗前后从每位参与者收集泪液样本，并使用基于微球的免疫分析测定7种细胞因子（IL-2、IL-6、IL-8、IL-10、IL-17A、TNF-α和ICAM-1）的泪液浓度。使用Wilcoxon检验分析治疗前和治疗后泪液样本之间的差异。
在基线时，反应者和无反应者之间在眼部症状或细胞因子水平上未观察到显著差异。局部治疗1个月后，反应者的眼表参数（包括眼表疾病指数、美国国立卫生研究院眼部评分、最佳矫正视力、角膜荧光素染色评分和荧光素泪膜破裂时间）显著改善，而无反应者则未改善。此外，无反应者中没有细胞因子表现出显著变化，而反应患者的泪液中IL-6（P = 0.031）和IL-8（P = 0.037）水平显著降低。
我们的结果表明，泪液中IL-以及IL-8水平在局部oGVHD治疗后发生了显著变化。 6
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