Horie Yoshifumi, Chiba Takashi
Research Center for Inland Seas, Kobe University, Kobe, Japan.
Department of Environmental and Symbiotic Science, Rakuno Gakuen University, Hokkaido, Japan.
Environ Toxicol Chem. 2023 Mar;42(3):673-678. doi: 10.1002/etc.5552. Epub 2023 Jan 30.
Japanese medaka is specified as a model fish in the test guidelines of the Organisation for Economic Co-operation and Development. Recently, populations of Japanese medaka in Japan were divided into two species, the northern Oryzias sakaizumii and the southern O. latipes. Previously, we reported that induction concentrations for sex reversal by exposure to 17α-methyltestosterone differed significantly between these two species, indicating that they respond differently to endocrine-disrupting chemica. In the present study, we examined the effects of exposure to two more endocrine-disrupting chemicals (bisphenol A and 17β-trenbolone) in O. sakaizumii, and compared the results with those previously reported for O. latipes. Exposure to both bisphenol A and 17β-trenbolone induced testis-ova formation or sex reversal in O. sakaizumii. Exposure to 17β-trenbolone also increased expression of gonadal soma-derived factor (gsdf). Least-observed-effect concentrations for gonadal sex differentiation and gsdf expression were lower for O. latipes than for O. sakaizumii after exposure to bisphenol A, and were lower for O. sakaizumii than for O. latipes after exposure to 17β-trenbolone. These results demonstrate that O. sakaizumii and O. latipes respond differently to androgenic and estrogenic endocrine-disrupting chemicals. Environ Toxicol Chem 2023;42:673-678. © 2022 SETAC.
日本青鳉被经济合作与发展组织的测试指南指定为模式鱼类。最近，日本的日本青鳉种群被分为两个物种，北部的坂井氏青鳉（Oryzias sakaizumii）和南部的青鳉（O. latipes）。此前，我们报道过，暴露于17α-甲基睾酮后，这两个物种性逆转的诱导浓度存在显著差异，这表明它们对内分泌干扰化学物质的反应不同。在本研究中，我们检测了坂井氏青鳉暴露于另外两种内分泌干扰化学物质（双酚A和17β-群勃龙）的影响，并将结果与之前报道的青鳉的结果进行了比较。暴露于双酚A和17β-群勃龙均诱导了坂井氏青鳉睾丸-卵巢的形成或性逆转。暴露于17β-群勃龙还增加了性腺体细胞衍生因子（gsdf）的表达。暴露于双酚A后，青鳉性腺性别分化和gsdf表达的最低观察效应浓度低于坂井氏青鳉，而暴露于17β-群勃龙后，坂井氏青鳉的最低观察效应浓度低于青鳉。这些结果表明，坂井氏青鳉和青鳉对雄激素和雌激素类内分泌干扰化学物质的反应不同。《环境毒理学与化学》2023年；42:673 - 678。© 2022 SETAC。
