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[小鼠非手术胚胎移植]

[Nonsurgical embryo transplantation in mice].

作者信息

Zubin M N, Veprintsev B N

出版信息

Ontogenez. 1987 Jul-Aug;18(4):434-7.

PMID:3658310
Abstract

Nonsurgical transplantation of the mouse embryos at the blastocyst stage into the uterus was performed using a device of original construction. The use of this device made it possible to exclude the loss of embryos, to reduce the amount of culture medium introduced together with the embryos, to lessen the damage of genital tract and ensured guaranteed "ejection" of embryos into the uterus. The females of the third day of pregnancy were used of recipients. Transplantation was performed between the mice of strains CBA/Lac, C57BL/6 and tetrahybrid CBWA. The level of newborn attained 65% of the number of transplanted blastocysts.

摘要

使用一种原创构造的装置将囊胚期小鼠胚胎非手术移植到子宫中。该装置的使用能够避免胚胎丢失,减少与胚胎一同引入的培养基量,减轻生殖道损伤,并确保胚胎能可靠地“植入”子宫。使用怀孕第三天的雌性小鼠作为受体。在CBA/Lac、C57BL/6品系小鼠和四元杂种CBWA之间进行移植。新生小鼠的数量达到移植囊胚数量的65%。

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