Örsten Serra, Baysal İpek, Akdoğan Neslihan, İnal Neşe, Bostan Ecem, Çiftçi Samiye Yabanoğlu, Akyön Yakut
Hacettepe University, Vocational School of Health Services, Ankara, Turkey.
Hacettepe University, Vocational School of Health Services, Ankara, Turkey.
Exp Parasitol. 2023 Feb;245:108453. doi: 10.1016/j.exppara.2022.108453. Epub 2022 Dec 28.
Blastocystis spp. has been proposed as a possible cause of extraintestinal clinical signs such as urticaria pathogenesis.
The aim of this study was to investigate the differences between microRNA (miRNA) expression profiles of Chronic spontaneous urticaria (CSU) patients in the presence or absence of Blastocystis spp. as well as healthy controls. Additionally, cellular pathways which are affected in the presence of Blastocystis spp. were identified.
Twenty patients diagnosed with CSU were enrolled in the study and divided into equally two groups according to the presence of Blastocystis spp. Besides, six healthy individuals were included in the study. The expression profiles of 372 human-derived miRNAs have been investigated in serum samples from CSU patients and healthy controls with miScript miRNA PCR Array Human miRBase Profiler.
Compared to Blastocystis-negative (BN)-CSU patients, expression of 3 miRNAs (hsa-miR-3183, hsa-miR-4469, hsa-miR-5191) were found to be downregulated by at least two-fold (p < 0.05) in Blastocystis-positive (BP)-CSU patients. Additionally, the miRNA expression profiles of six healthy individuals (n = 3 Blastocystis-positive, n = 3 Blastocystis-negative) were analyzed and it was determined that the expressions of 7 miRNAs (hsa-miR-4661-5p, hsa-miR-4666a-5p, hsa-miR-4803, hsa-miR-5587-5p, hsa-miR-4500, hsa-miR-5680, hsa-miR-382-3p) increased at least 3-fold in the serum of individuals with Blastocystis-positive compared to Blastocystis-negative subjects. Most down-regulated miRNAs, in BP-CSU patients, affect cell adhesion molecules (CAMs), and signaling pathways therefore, Blastocystis spp. presence may influence the clinical presentation of urticaria by leading to unbalanced immunity. In addition, Blastocystis spp. presence may be influenced TGF- β signaling pathway through altered miRNAs and may be laying the groundwork for the development of CSU in healthy individuals.
As a consequence, this is the first report to show that the miRNA expression profile is affected by the presence of Blastocystis spp. Further miRNA-based studies are needed in order to enlighten the exact underlying molecular mechanisms of the relationship between Blastocystis spp. and CSU.
芽囊原虫属被认为可能是诸如荨麻疹发病机制等肠外临床症状的病因。
本研究旨在调查慢性自发性荨麻疹（CSU）患者在存在或不存在芽囊原虫属情况下以及健康对照者之间微小RNA（miRNA）表达谱的差异。此外，还确定了在存在芽囊原虫属情况下受影响的细胞途径。
20例被诊断为CSU的患者纳入本研究，并根据是否存在芽囊原虫属平均分为两组。此外，6名健康个体也纳入研究。使用miScript miRNA PCR Array Human miRBase Profiler对CSU患者和健康对照者的血清样本中372种人源miRNA的表达谱进行了研究。
与芽囊原虫属阴性（BN）的CSU患者相比，发现芽囊原虫属阳性（BP）的CSU患者中3种miRNA（hsa-miR-3183、hsa-miR-4469、hsa-miR-5191）的表达下调至少两倍（p<0.05）。此外，分析了6名健康个体（3名芽囊原虫属阳性，3名芽囊原虫属阴性）的miRNA表达谱，确定与芽囊原虫属阴性个体相比，芽囊原虫属阳性个体血清中7种miRNA（hsa-miR-4661-5p、hsa-miR-4666a-5p、hsa-miR-4803、hsa-miR-5587-5p、hsa-miR-4500、hsa-miR-5680、hsa-miR-382-3p）的表达增加至少3倍。在BP-CSU患者中，下调最明显的miRNA影响细胞黏附分子（CAMs）和信号通路，因此，芽囊原虫属的存在可能通过导致免疫失衡影响荨麻疹的临床表现。此外，芽囊原虫属的存在可能通过改变miRNA影响TGF-β信号通路，并可能为健康个体中CSU的发展奠定基础。
因此，这是第一份表明miRNA表达谱受芽囊原虫属存在影响的报告。需要进一步开展基于miRNA的研究，以阐明芽囊原虫属与CSU之间关系的确切潜在分子机制。
