Department of Dermatology, No. 1 Hospital of China Medical University, 155 North Nanjing Street, Shenyang 110001, China.
Acta Derm Venereol. 2019 May 1;99(6):571-578. doi: 10.2340/00015555-3149.
Chronic spontaneous urticaria (CSU) is a common skin disorder associated with autoimmunity. MicroRNAs (miRNAs) are endogenous noncoding RNA molecules reported to be potential biomarkers for some autoimmune diseases. In this study, we investigated the association of miRNAs with CSU. A quantitative PCR (qPCR)-based array was generated from sera as obtained from 20 active CSU patients and 20 healthy controls. Upregulated or downregulated miRNAs were validated by reverse transcription qPCR in sera from 59 active CSU patients and 58 healthy controls. The expression of miR-125a-5p was significantly upregulated in CSU sera and serum levels of CCL17 were also significantly increased in CSU patients. Serum miR-125a-5p expressions were found to be further upregulated in refractory CSU cases (n = 10). In 12 CSU patients in remission, serum miR-125a-5p expression and CCL17 levels were significantly decreased as compared with that obtained in active phase patients. These results indicated that miR-125a-5p and CCL17 can serve as potential serum biomarkers for CSU.
慢性自发性荨麻疹（CSU）是一种与自身免疫相关的常见皮肤疾病。microRNAs（miRNAs）是内源性非编码 RNA 分子，据报道，它们可能是一些自身免疫性疾病的潜在生物标志物。在本研究中，我们研究了 miRNAs 与 CSU 的关联。从 20 例活动性 CSU 患者和 20 例健康对照者的血清中生成了基于定量 PCR（qPCR）的阵列。在 59 例活动性 CSU 患者和 58 例健康对照者的血清中通过逆转录 qPCR 验证了上调或下调的 miRNAs。miR-125a-5p 在 CSU 血清中的表达显著上调，CCL17 的血清水平在 CSU 患者中也显著升高。发现难治性 CSU 病例（n=10）的血清 miR-125a-5p 表达进一步上调。在 12 例缓解期 CSU 患者中，与活动期患者相比，血清 miR-125a-5p 表达和 CCL17 水平显著降低。这些结果表明，miR-125a-5p 和 CCL17 可作为 CSU 的潜在血清生物标志物。
