Beijing Key Laboratory for Bioengineering and Sensing Technology, Research Center for Bioengineering and Sensing Technology, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, 30 Xueyuan Road, Beijing 100083, PR China.
Beijing Key Laboratory for Bioengineering and Sensing Technology, Research Center for Bioengineering and Sensing Technology, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, 30 Xueyuan Road, Beijing 100083, PR China; Marshall Laboratory of Biomedical Engineering, School of Biomedical Engineering, Health Science Centre, Shenzhen University, Shenzhen 518071, PR China.
Acta Biomater. 2023 Mar 1;158:252-265. doi: 10.1016/j.actbio.2022.12.049. Epub 2022 Dec 27.
Antibiotic resistance of bacteria and persistent inflammation are critical challenges in treating bacteria infected wounds. Thus, it is urgent to develop versatile wound dressings that possess high-efficiency antibacterial performance and inflammation regulation. Herein, we have successfully constructed a hydrogel wound dressing consisting of the bimetallic metal-organic framework (MOF) loaded with glucose oxidase (GOx), termed as MOF(Fe-Cu)/GOx-polyacrylamide (PAM) gel. Hydrogel dressings can provide an efficient cascade-catalyzed system to accelerate wound healing via synergistic antibacterial and inflammatory modulation. Importantly, the catalytic property of the bimetallic MOF(Fe-Cu) is about five times that of the monometallic MOF(Fe). Based on such a cascade-catalyzed system, the abundant gluconic acid and HO can be continuously produced by decomposing glucose via GOx. Such gluconic acid can notably improve the peroxidase performance of MOF(Fe-Cu), which can further efficiently decompose HO to achieve the antibacterial. Meanwhile, MOF (Fe Cu)/GOx PAM gel can induce macrophages to change into an M2 phenotype, which can accelerate the transformation of the wound microenvironment to a remodeling state and then accelerate angiogenesis and neurogenesis. This work provides multifunctional bioactive materials for accelerating wound healing and will have great potential in clinical applications. STATEMENT OF SIGNIFICANCE: Antibiotic resistance and persistent inflammation are still the critical reasons for the slow healing of bacteria infected wounds. Herein, we prepared a hydrogel wound dressing composed of bimetallic metal organic framework (MOF) loaded with glucose oxidase (GOx). The catalytic activity of the bimetallic MOF(Fe-Cu) is significantly enhanced due to doping of copper, which makes it possess outstanding antibacterial ability based on cascade catalysis. Such dressing can promote the remodeling of inflammatory immunity by regulating macrophage polarization to suppress over-reactive inflammation, further accelerating the healing of bacteria-infected wounds. This study provides an innovative and effective way to accelerate the healing of bacteria infected wound by combining bacteria killing and inflammation modulation.
细菌的抗生素耐药性和持续的炎症是治疗细菌感染伤口的关键挑战。因此，迫切需要开发多功能的伤口敷料，这种敷料应具有高效的抗菌性能和炎症调节功能。在此，我们成功构建了一种水凝胶伤口敷料，它由负载葡萄糖氧化酶（GOx）的双金属金属有机骨架（MOF）组成，称为 MOF(Fe-Cu)/GOx-聚丙烯酰胺（PAM）凝胶。水凝胶敷料可以通过协同抗菌和炎症调节提供一种高效的级联催化系统，从而加速伤口愈合。重要的是，双金属 MOF(Fe-Cu)的催化性能大约是单金属 MOF(Fe)的五倍。基于这种级联催化系统，通过 GOx 分解葡萄糖可以持续产生丰富的葡萄糖酸和 HO。这种葡萄糖酸可以显著提高 MOF(Fe-Cu)的过氧化物酶性能，从而进一步有效地分解 HO 以实现抗菌作用。同时，MOF(Fe-Cu)/GOx-PAM 凝胶可以诱导巨噬细胞转变为 M2 表型，从而加速伤口微环境向重塑状态的转变，进而加速血管生成和神经发生。这项工作为加速伤口愈合提供了多功能的生物活性材料，在临床应用中具有巨大的潜力。
抗生素耐药性和持续的炎症仍然是导致细菌感染伤口愈合缓慢的关键原因。在此，我们制备了一种由负载葡萄糖氧化酶（GOx）的双金属金属有机骨架（MOF）组成的水凝胶伤口敷料。由于掺杂铜，双金属 MOF(Fe-Cu)的催化活性显著增强，这使其基于级联催化具有出色的抗菌能力。这种敷料可以通过调节巨噬细胞极化来促进炎症免疫的重塑，以抑制过度活跃的炎症，从而进一步加速细菌感染伤口的愈合。本研究为通过杀菌和炎症调节相结合来加速细菌感染伤口的愈合提供了一种创新且有效的方法。
