Key Laboratory of Livestock and Poultry Multi-omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, PR China; Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan, 611130, PR China.
Key Laboratory of Livestock and Poultry Multi-omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, PR China; Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan, 611130, PR China.
Poult Sci. 2023 Mar;102(3):102428. doi: 10.1016/j.psj.2022.102428. Epub 2022 Dec 15.
Our previous study described the mechanism of goose fatty liver formation from cell culture and transcriptome. However, how lipidome of goose liver response to overfeeding is unclear. In this study, we used the same batch of geese (control group and corn flour overfeeding group) to explore the lipidome changes and underlying metabolic mechanisms of goose fatty liver formation. Liquid chromatography-mass spectrometry (LC-MS) was provided to lipidome detection. Liver lipidomics profiles analysis was performed by principal component analysis (PCA), partial least squares-discriminant analysis (PLS-DA) and orthogonal partial least squares-discriminant analysis (OPLS-DA), different lipids were identified and annotated, and the enriched metabolic pathways were showed. The results of PCA, PLS-DA, and OPLS-DA displayed a clear separation and discrimination between control group and corn flour overfeeding group. Two hundred and fifty-one different lipids were yielded, which were involved in triglyceride (TG), diglyceride (DG), phosphatidic acids (PA), phosphatidylinositols (PI), phosphatidylethanolamines (PE), phosphatidylcholines (PC), lyso-phosphatidylcholines (LPC), monogalactosylmonoacylglycerol (MGMG), sphingolipids (SM), ceramides (Cer), and hexaglycosylceramides (Hex1Cer). Different lipids were enriched in glycerophospholipid metabolism, glycerolipid metabolism, phosphatidylinositol signaling system, inositol phosphate metabolism, glycosylphosphatidylinositol (GPI)-anchor biosynthesis and sphingolipid metabolism. In conclusion, this is the first report describing the goose fatty liver formation from lipidomics, this study might provide some insights into the underlying glucolipid metabolism disorders in the process of fatty liver formation.
我们之前的研究从细胞培养和转录组学方面描述了鹅脂肪肝形成的机制。然而，鹅肝脂质组如何对过量喂养做出反应尚不清楚。在这项研究中，我们使用了同一批鹅（对照组和玉米粉过量喂养组）来探索鹅脂肪肝形成过程中脂质组的变化及其潜在的代谢机制。采用液相色谱-质谱法（LC-MS）进行脂质组检测。通过主成分分析（PCA）、偏最小二乘判别分析（PLS-DA）和正交偏最小二乘判别分析（OPLS-DA）对肝脂质组学图谱进行分析，鉴定和注释不同的脂质，并显示富集的代谢途径。PCA、PLS-DA 和 OPLS-DA 的结果显示，对照组和玉米粉过量喂养组之间有明显的分离和区分。得出了 251 种不同的脂质，它们涉及甘油三酯（TG）、二甘油酯（DG）、磷脂酸（PA）、磷脂酰肌醇（PI）、磷脂酰乙醇胺（PE）、磷脂酰胆碱（PC）、溶血磷脂酰胆碱（LPC）、单半乳糖基单酰基甘油（MGMG）、鞘脂（SM）、神经酰胺（Cer）和六糖苷神经酰胺（Hex1Cer）。不同的脂质在甘油磷脂代谢、甘油脂代谢、磷脂酰肌醇信号系统、肌醇磷酸盐代谢、糖基磷脂酰肌醇（GPI）-锚生物合成和鞘脂代谢中富集。总之，这是首次从脂质组学角度描述鹅脂肪肝形成的研究，本研究可能为脂肪肝形成过程中潜在的糖脂代谢紊乱提供一些见解。
