Kansas State University, Department of Animal Sciences and Industry, Manhattan, Kansas 66506, USA.
Kansas State University, Division of Biology, Manhattan, Kansas, 66506, USA.
Meat Sci. 2023 Apr;198:109091. doi: 10.1016/j.meatsci.2022.109091. Epub 2022 Dec 29.
The objective of this study was to elucidate the effect of phospholipase A2 (PLA2) and a PLA2 antibody (aPLA2) on phospholipid (PL) hydrolysis in beef and to understand how the altered PL composition may affect lipid oxidation and antioxidant capacity of beef in an in vitro system. Various combinations of PLA2 and aPLA2 were introduced to a beef liposome model system and exposed to a retail display. The PL and free fatty acid (FFA) profiles, antioxidant capacity and lipid oxidation were measured for the liposome system. Key PL classes were reduced and the release of polyunsaturated FFAs was increased with the inclusion of PLA2 in the treatments (P < 0.05). There was no inhibition of PL hydrolysis with the addition of aPLA2. PLA2 showed strong antioxidant capacity in the liposome system (P < 0.01), but lipid oxidation still increased in samples treated with PLA2 throughout the retail display (P < 0.01). Finally, aPLA2 treatments demonstrated potential to decrease lipid oxidation (P < 0.01).
本研究旨在阐明磷脂酶 A2 (PLA2) 和 PLA2 抗体 (aPLA2) 对牛肉中磷脂 (PL) 水解的影响，并了解在体外系统中，改变的 PL 组成如何影响牛肉的脂质氧化和抗氧化能力。将各种组合的 PLA2 和 aPLA2 引入牛肉脂质体模型系统，并暴露于零售展示中。对脂质体系统进行 PL 和游离脂肪酸 (FFA) 谱、抗氧化能力和脂质氧化的测量。PL 的关键类别减少，多不饱和 FFAs 的释放增加，PLA2 的加入处理中（P < 0.05）。添加 aPLA2 并没有抑制 PL 水解。PLA2 在脂质体系统中表现出很强的抗氧化能力（P < 0.01），但在整个零售展示过程中，用 PLA2 处理的样品中的脂质氧化仍然增加（P < 0.01）。最后，aPLA2 处理表现出降低脂质氧化的潜力（P < 0.01）。
