Department of Cariology, Restorative Sciences, and Endodontics, University of Michigan School of Dentistry, 1011 N University Avenue, Ann Arbor, MI, 48109, USA; Department of Pulp Biology and Endodontics, Division of Oral Health Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), Tokyo, Japan.
Department of Cariology, Restorative Sciences, and Endodontics, University of Michigan School of Dentistry, 1011 N University Avenue, Ann Arbor, MI, 48109, USA.
J Oral Biosci. 2023 Mar;65(1):132-135. doi: 10.1016/j.job.2022.12.003. Epub 2022 Dec 29.
Apical periodontitis is an inflammatory disease involving lesions located within the jawbone. Histological evaluations generally require decalcification and sectioning, which has limited our understanding of the three-dimensional (3D) organization and spatial distribution of different immune cell types in these lesions. A recently developed technique combining tissue clearing and whole-mount immunofluorescent labeling allows us to acquire such information from the deep tissue without sectioning. However, whole-mount immunofluorescent labeling in the jawbone requires further development. Here we provide a straightforward and efficient protocol to achieve 3D immunofluorescent imaging of murine periapical lesions.
根尖周炎是一种涉及颌骨内病变的炎症性疾病。组织学评估通常需要脱钙和切片，这限制了我们对这些病变中不同免疫细胞类型的三维（3D）组织和空间分布的理解。最近开发的一种结合组织透明化和全组织免疫荧光标记的技术，使我们能够在不进行切片的情况下从深部组织中获取这些信息。然而，颌骨的全组织免疫荧光标记需要进一步开发。在这里，我们提供了一种简单有效的方案，用于实现小鼠根尖周病变的 3D 免疫荧光成像。
