Wang Yuzhen, Xu Rui, Luo Gaoxing, Wu Jun
Institute of Burn Research, Southwest Hospital, State Key Laboratory of Trauma, Burns, and Combined Injury, Chongqing Key Laboratory for Diseases Proteomics, the Third Military Medical University, Chongqing, 400038, China.
Front Med. 2015 Mar;9(1):30-45. doi: 10.1007/s11684-014-0337-z. Epub 2014 Jun 20.
Three-dimensional (3D) image reconstruction technologies can reveal previously hidden microstructures in human tissue. However, the lack of ideal, non-destructive cross-sectional imaging techniques is still a problem. Despite some drawbacks, histological sectioning remains one of the most powerful methods for accurate high-resolution representation of tissue structures. Computer technologies can produce 3D representations of interesting human tissue and organs that have been serial-sectioned, dyed or stained, imaged, and segmented for 3D visualization. 3D reconstruction also has great potential in the fields of tissue engineering and 3D printing. This article outlines the most common methods for 3D tissue section reconstruction. We describe the most important academic concepts in this field, and provide critical explanations and comparisons. We also note key steps in the reconstruction procedures, and highlight recent progress in the development of new reconstruction methods.
三维（3D）图像重建技术能够揭示人体组织中先前隐藏的微观结构。然而，缺乏理想的、非破坏性的横截面成像技术仍是一个问题。尽管存在一些缺点，但组织切片仍然是精确高分辨率呈现组织结构的最强大方法之一。计算机技术可以对经过连续切片、染色、成像以及为三维可视化进行分割的人体组织和器官生成三维表示。三维重建在组织工程和3D打印领域也具有巨大潜力。本文概述了三维组织切片重建的最常用方法。我们描述了该领域最重要的学术概念，并提供了关键的解释和比较。我们还指出了重建过程中的关键步骤，并强调了新重建方法开发方面的最新进展。
