Yue Huifeng, Yang Xiaowen, Wu Xiaoyun, Tian Yuchai, Xu Pengchong, Sang Nan
College of Environment and Resource, Research Center of Environment and Health, Shanxi University, Taiyuan, Shanxi 030006, PR China.
College of Environment and Resource, Research Center of Environment and Health, Shanxi University, Taiyuan, Shanxi 030006, PR China.
Environ Pollut. 2023 Feb 15;319:120980. doi: 10.1016/j.envpol.2022.120980. Epub 2022 Dec 29.
The ban on bisphenol A (BPA) has led to a rapid increase in the use of BPA analogs, and they are increasingly being detected in the natural environment and biological organisms. Studies have pointed out that BPA analogs can lead to adverse health outcomes. However, their interference with ovarian tissue has not been fully elucidated. In this study, seven- to eight-week-old CD-1 mice were exposed to corn oil containing 300 μg/kg/day bisphenol B (BPB) or bisphenol AF (BPAF) through oral gavage, and ovarian tissues were collected at 14 and 28 days of exposure. Ovarian toxicity was evaluated by the ovarian index, ovarian area, and follicle number. mRNA-seq was used to identify differentially expressed genes (DEGs) and infer the association of DEGs with ovarian diseases. BPB or BPAF exposure induced morphological changes in ovarian tissue in CD-1 mice. In addition, Gene Ontology (GO) analysis revealed disturbances in biological processes (BP) associated with steroid biosynthetic process (GO:0006694) and cellular calcium ion homeostasis (GO:0006874). Subsequently, regulatory networks of BPA analogs (BPB or BPAF)-DEGs-ovarian diseases were constructed. Importantly, the expression levels of DEGs and transcription factors (TFs) associated with ovarian disease were altered. BPB or BPAF exposure causes damage to ovarian morphology through the synergistic effects of multiple biological processes and may be associated with altered mRNA expression profiles as a risk factor for ovarian diseases.
双酚A（BPA）禁令导致双酚A类似物的使用迅速增加，并且它们在自然环境和生物体内被检测到的频率越来越高。研究指出，双酚A类似物可导致不良健康后果。然而，它们对卵巢组织的干扰尚未完全阐明。在本研究中，通过口服灌胃将7至8周龄的CD-1小鼠暴露于含有300μg/kg/天双酚B（BPB）或双酚AF（BPAF）的玉米油中，并在暴露14天和28天时收集卵巢组织。通过卵巢指数、卵巢面积和卵泡数量评估卵巢毒性。使用mRNA测序来鉴定差异表达基因（DEG）并推断DEG与卵巢疾病的关联。BPB或BPAF暴露诱导了CD-1小鼠卵巢组织的形态变化。此外，基因本体（GO）分析揭示了与类固醇生物合成过程（GO:0006694）和细胞钙离子稳态（GO:0006874）相关的生物过程（BP）受到干扰。随后，构建了双酚A类似物（BPB或BPAF）-DEG-卵巢疾病的调控网络。重要的是，与卵巢疾病相关的DEG和转录因子（TF）的表达水平发生了改变。BPB或BPAF暴露通过多种生物过程的协同作用对卵巢形态造成损害，并且可能与作为卵巢疾病风险因素的mRNA表达谱改变有关。
