Daneshyar Saeed, Tavoosidana Gholamreza, Bahmani Mahdi, Basir Saeed Shokati, Delfan Maryam, Laher Ismail, Saeidi Ayoub, Granacher Urs, Zouhal Hassane
Department of Physical Education, Faculty of Humanities, Ayatollah Alozma Boroujerdi University, Lorestan, Iran; Department of Physical Education, Hamedan University of Technology, Hamedan, Iran.
Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Life Sci. 2023 Feb 1;314:121335. doi: 10.1016/j.lfs.2022.121335. Epub 2022 Dec 29.
The effects of nutrition and exercise on autophagy are not well studied. This study aimed to investigate the combined effects of high-fat diets (HFD) and exercise training (ET) on autophagy in white adipose tissue of mice.
Male C57BL/6 mice were assigned into four groups of 7 mice per group: (1) Control, (2) high-fat diet-induced obesity (HFD-Ob), (3) exercise training (ET), and (4) high-fat diet with exercise training (HFD-ET). The HFD-Ob group was fed a high-fat diet for 14 weeks, while the ET group continuously ran on a treadmill for five sessions per week for seven weeks, and the HFD-ET group had both HFD and exercise training. qReal-time-PCR and western blot were used to measure the mRNA and protein levels of autophagy markers in white adipose tissue.
Mice from the HFD group showed higher levels in autophagy-related gene5 (ATG5, p = 0.04), ATG7 (p = 0.002), cathepsin B (CTSB, p = 0.0004), LC3-II (p = 0.03) than control. Mice in the ET group displayed higher levels of genes for ATG7 (p = 0.0003), microtubule-associated protein1-light chain 3 (LC3, p = 0.05), lysosome-associated membrane protein 2 (LAMP2, p = 0.04) and cathepsin L (CTSL, p = 0.03) than control. Mice from the HFD-ET group had higher levels of genes for ATG7 (p = 0.05) and CTSL (p = 0.043) and lower levels of genes for CTSB (p = 0.045) compared to the HFD group and lower levels of LAMP2 (p = 0.02) compared to the ET group.
There were increases in autophagosome formation in the white adipose tissue from mice in the HFD and ET groups. A combination of HFD and ET enhances autophagosome formation and modulates lysosomal degradation in white adipose tissue.
营养与运动对自噬的影响尚未得到充分研究。本研究旨在探讨高脂饮食（HFD）和运动训练（ET）对小鼠白色脂肪组织自噬的联合作用。
将雄性C57BL/6小鼠分为四组，每组7只：（1）对照组，（2）高脂饮食诱导肥胖组（HFD-Ob），（3）运动训练组（ET），（4）高脂饮食加运动训练组（HFD-ET）。HFD-Ob组给予高脂饮食14周，ET组每周在跑步机上连续跑5次，共7周，HFD-ET组同时进行高脂饮食和运动训练。采用qReal-time-PCR和蛋白质印迹法检测白色脂肪组织中自噬标志物的mRNA和蛋白质水平。
HFD组小鼠的自噬相关基因5（ATG5，p = 0.04）、ATG7（p = 0.002）、组织蛋白酶B（CTSB，p = 0.0004）、LC3-II（p = 0.03）水平高于对照组。ET组小鼠的ATG7（p = 0.0003）、微管相关蛋白1轻链3（LC3，p = 0.05）、溶酶体相关膜蛋白2（LAMP2，p = 0.04）和组织蛋白酶L（CTSL，p = 0.03）基因水平高于对照组。与HFD组相比，HFD-ET组小鼠的ATG7（p = 0.05）和CTSL（p = 0.043）基因水平较高，CTSB（p = 0.045）基因水平较低；与ET组相比，LAMP2（p = 0.02）基因水平较低。
HFD组和ET组小鼠白色脂肪组织中的自噬体形成增加。高脂饮食与运动训练相结合可增强白色脂肪组织中的自噬体形成并调节溶酶体降解。
