Endometriosis Research Center, Iran University of Medical Sciences, Tehran, Iran.
Department of Physiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Biomed Res Int. 2022 Dec 21;2022:1111777. doi: 10.1155/2022/1111777. eCollection 2022.
Studies suggest that ovarian hyperstimulation syndrome (OHSS) can be treated by reducing the level of vascular endothelial growth factor (VEGF). However, due to the side effects of commercially available VEGF-reducing drugs, they can be ruled out as a suitable treatment for OHSS; therefore, researchers are looking for new medications to treat OHSS. This study is aimed at investigating the effects of cannabidiol (CBD) in an OHSS model and to evaluate its efficacy in modulating the angiogenesis pathway and gene expression. For this purpose, 32 female mice were randomly divided into four groups (eight mice per group): control group, group 2 with OHSS induction, group 3 receiving 32 nmol of dimethyl sulfoxide after OHSS induction, and group 4 receiving 30 mg/kg of CBD after OHSS induction. The animals' body weight, ovarian weight, vascular permeability (VP), and ovarian follicle count were measured, and the levels of gene and protein expression in the peritoneal fluid were assessed. Based on the results, CBD decreased the body and ovarian weights, VP, and corpus luteum number compared to the OHSS group ( < 0.05). The peritoneal gene and protein expression levels reduced in the CBD group compared to the OHSS group ( < 0.05). Also, CBD caused OHSS alleviation by suppressing expression and VP. Overall, CBD downregulated gene expression and improved VP in OHSS.
研究表明，卵巢过度刺激综合征（OHSS）可以通过降低血管内皮生长因子（VEGF）水平来治疗。然而，由于市售的 VEGF 降低药物的副作用，它们不能作为 OHSS 的合适治疗方法；因此，研究人员正在寻找新的药物来治疗 OHSS。本研究旨在探讨大麻二酚（CBD）在 OHSS 模型中的作用，并评估其在调节血管生成途径和基因表达方面的疗效。为此，将 32 只雌性小鼠随机分为四组（每组 8 只）：对照组、OHSS 诱导组 2、OHSS 诱导后给予 32nmol 二甲基亚砜的组 3 和 OHSS 诱导后给予 30mg/kg CBD 的组 4。测量动物的体重、卵巢重量、血管通透性（VP）和卵巢卵泡计数，并评估腹腔液中基因和蛋白表达水平。基于结果，与 OHSS 组相比，CBD 降低了体质量和卵巢质量、VP 和黄体数量（<0.05）。与 OHSS 组相比，CBD 组腹腔液中的基因和蛋白表达水平降低（<0.05）。此外，CBD 通过抑制基因表达和 VP 来减轻 OHSS。总体而言，CBD 下调了 OHSS 中的基因表达并改善了 VP。
