Department of Civil, Construction and Environmental Engineering, North Dakota State University, Fargo, ND, USA.
Ann Biomed Eng. 2023 Jun;51(6):1199-1215. doi: 10.1007/s10439-022-03123-8. Epub 2023 Jan 2.
We investigated the potential correlation between the fluid shear stress and the proliferation of bone prostate cancer cells on the surface of nanoclay-based scaffolds in a perfusion bioreactor. Human mesenchymal stem cells (hMSCs) were seeded on the scaffolds to initiate bone growth. After 23 days, prostate cancer cells (MDAPCa2b) were cultured on top of the osteogenically differentiated hMSCs. The scaffolds were separated into two groups subjected to two distinct conditions: (i) static (no flow); and (ii) dynamic (with flow) conditions to recapitulate bone metastasis of prostate cancer. Based on measured data, Computational Fluid Dynamics (CFD) models were constructed to determine the velocity and shear stress distributions on the scaffold surface. Our experimental results show distinct differences in the growth pattern of hMSCs and MDAPCa2b cells between the static and dynamic conditions. Our computational results further suggest that the dynamic flow leads to drastic change in cell morphology and tumorous distribution. Our work points to a strong correlation between tumor growth and local interstitial flows in bones.
我们研究了在灌注生物反应器中，纳米粘土基支架表面的流体切应力与前列腺癌细胞增殖之间的潜在相关性。将人骨髓间充质干细胞 (hMSC) 接种在支架上以启动骨生长。23 天后，在成骨分化的 hMSC 上培养前列腺癌细胞 (MDAPCa2b)。将支架分为两组，分别处于两种不同的条件下：（i）静态（无流动）；和（ii）动态（有流动），以再现前列腺癌的骨转移。根据测量数据，构建了计算流体动力学 (CFD) 模型，以确定支架表面的速度和剪切应力分布。我们的实验结果表明，静态和动态条件下 hMSC 和 MDAPCa2b 细胞的生长模式有明显差异。我们的计算结果进一步表明，动态流动会导致细胞形态和肿瘤分布的剧烈变化。我们的工作表明，肿瘤生长与骨骼内局部间质流之间存在很强的相关性。
