• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

切应力诱导人骨髓间充质干细胞的成骨分化。

Shear stress induces osteogenic differentiation of human mesenchymal stem cells.

机构信息

Department of Physiology & Biophysics, University of Illinois at Chicago, Chicago, IL 60612, USA.

出版信息

Regen Med. 2010 Sep;5(5):713-24. doi: 10.2217/rme.10.60.

DOI:10.2217/rme.10.60
PMID:20868327
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4093787/
Abstract

AIM

To determine whether fluid flow-induced shear stress affects the differentiation of bone marrow-derived human mesenchymal stem cells (hMSCs) into osteogenic cells.

MATERIALS & METHODS: hMSCs cultured with or without osteogenic differentiation medium were exposed to fluid flow-induced shear stress and analyzed for alkaline phosphatase activity and expression of osteogenic genes.

RESULTS

Immediately following shear stress, alkaline phosphatase activity in osteogenic medium was significantly increased. At days 4 and 8 of culture the mRNA expression of bone morphogenetic protein-2 and osteopontin was significantly higher in hMSCs subjected to shear stress than those cultured in static conditions. However, hMSCs cultured in osteogenic differentiation medium were less responsive in gene expression of alkaline phosphatase and bone morphogenetic protein-2.

CONCLUSION

These data demonstrate that shear stress stimulates hMSCs towards an osteoblastic phenotype in the absence of chemical induction, suggesting that certain mechanical stresses may serve as an alternative to chemical stimulation of stem cell differentiation.

摘要

目的

确定流体流动引起的切应力是否会影响骨髓来源的人骨髓间充质干细胞（hMSCs）向成骨细胞的分化。

材料与方法

将培养有或没有成骨分化培养基的 hMSCs 暴露于流体流动引起的切应力下，并分析碱性磷酸酶活性和成骨基因的表达。

结果

切应力后即刻，成骨培养基中的碱性磷酸酶活性显著增加。在培养的第 4 天和第 8 天，与静态培养条件相比，hMSCs 中的骨形态发生蛋白-2 和骨桥蛋白的 mRNA 表达明显更高。然而，在成骨分化培养基中培养的 hMSCs 在碱性磷酸酶和骨形态发生蛋白-2 的基因表达方面反应较弱。

结论

这些数据表明，在没有化学诱导的情况下，切应力刺激 hMSCs 向成骨细胞表型分化，这表明某些机械应力可能可作为干细胞分化的化学刺激的替代物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f40f/4093787/da640142b13a/nihms267788f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f40f/4093787/3f56e0663c66/nihms267788f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f40f/4093787/a2f9909e78b4/nihms267788f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f40f/4093787/31f61c5709a7/nihms267788f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f40f/4093787/33a5ba415d20/nihms267788f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f40f/4093787/a668ebaf23ed/nihms267788f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f40f/4093787/da640142b13a/nihms267788f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f40f/4093787/3f56e0663c66/nihms267788f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f40f/4093787/a2f9909e78b4/nihms267788f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f40f/4093787/31f61c5709a7/nihms267788f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f40f/4093787/33a5ba415d20/nihms267788f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f40f/4093787/a668ebaf23ed/nihms267788f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f40f/4093787/da640142b13a/nihms267788f6.jpg

相似文献

1
Shear stress induces osteogenic differentiation of human mesenchymal stem cells.切应力诱导人骨髓间充质干细胞的成骨分化。
Regen Med. 2010 Sep;5(5):713-24. doi: 10.2217/rme.10.60.
2
Molecular and functional expression of voltage-operated calcium channels during osteogenic differentiation of human mesenchymal stem cells.人骨髓间充质干细胞成骨分化过程中电压门控钙通道的分子与功能表达
J Bone Miner Res. 2005 Sep;20(9):1637-46. doi: 10.1359/JBMR.050521. Epub 2005 May 31.
3
Studies on culture and osteogenic induction of human mesenchymal stem cells under CO2-independent conditions.在 CO2 非依赖条件下对人骨髓间充质干细胞的培养和成骨诱导研究。
Astrobiology. 2013 Apr;13(4):370-9. doi: 10.1089/ast.2012.0922. Epub 2013 Apr 11.
4
Mechanisms for osteogenic differentiation of human mesenchymal stem cells induced by fluid shear stress.流体切应力诱导人骨髓间充质干细胞成骨分化的机制。
Biomech Model Mechanobiol. 2010 Dec;9(6):659-70. doi: 10.1007/s10237-010-0206-x. Epub 2010 Mar 23.
5
Influence of mechanical fluid shear stress on the osteogenic differentiation protocols for Equine adipose tissue-derived mesenchymal stem cells.机械流体剪切应力对马脂肪组织来源间充质干细胞成骨分化方案的影响
Acta Histochem. 2019 Apr;121(3):344-353. doi: 10.1016/j.acthis.2019.02.002. Epub 2019 Feb 23.
6
Different effects of intermittent and continuous fluid shear stresses on osteogenic differentiation of human mesenchymal stem cells.间歇和连续流体力对人骨髓间充质干细胞成骨分化的不同影响。
Biomech Model Mechanobiol. 2012 Mar;11(3-4):391-401. doi: 10.1007/s10237-011-0319-x. Epub 2011 Jun 2.
7
Synergistic effect of recombinant human bone morphogenic protein-7 and osteogenic differentiation medium on human bone-marrow-derived mesenchymal stem cells in vitro.重组人骨形态发生蛋白-7 与成骨分化培养基在体外对人骨髓间充质干细胞的协同作用。
Int Orthop. 2011 Dec;35(12):1889-95. doi: 10.1007/s00264-011-1247-1. Epub 2011 Apr 13.
8
Bioreactor strategy in bone tissue engineering: pre-culture and osteogenic differentiation under two flow configurations.骨组织工程中的生物反应器策略：两种流动状态下的预培养和成骨分化。
Tissue Eng Part A. 2012 Nov;18(21-22):2354-64. doi: 10.1089/ten.TEA.2011.0674. Epub 2012 Jul 19.
9
Chip-based comparison of the osteogenesis of human bone marrow- and adipose tissue-derived mesenchymal stem cells under mechanical stimulation.基于芯片的比较研究：在机械刺激下，来源于人骨髓和脂肪组织的间充质干细胞的成骨作用。
PLoS One. 2012;7(9):e46689. doi: 10.1371/journal.pone.0046689. Epub 2012 Sep 28.
10
Platelet-rich concentrate supports human mesenchymal stem cell proliferation, bone morphogenetic protein-2 messenger RNA expression, alkaline phosphatase activity, and bone formation in vitro: a mode of action to enhance bone repair.富血小板浓缩物在体外支持人间充质干细胞增殖、骨形态发生蛋白-2信使核糖核酸表达、碱性磷酸酶活性及骨形成：一种促进骨修复的作用模式。
J Orthop Trauma. 2008 Oct;22(9):595-604. doi: 10.1097/BOT.0b013e318188dbb7.

引用本文的文献

1
Shear Stress Regulates Osteogenic Differentiation of Human Dental Pulp Stem Cells via the p38 Pathway.剪切应力通过p38信号通路调控人牙髓干细胞的成骨分化。
Int J Mol Sci. 2025 Jun 13;26(12):5667. doi: 10.3390/ijms26125667.
2
Recapitulating the bone extracellular matrix through 3D bioprinting using various crosslinking chemistries.通过使用各种交联化学方法的3D生物打印来重现骨细胞外基质。
Front Bioeng Biotechnol. 2025 Jun 5;13:1506122. doi: 10.3389/fbioe.2025.1506122. eCollection 2025.
3
The Effects of Electrical Stimulation on a 3D Osteoblast Cell Model.

本文引用的文献

1
Short bouts of mechanical loading are as effective as dexamethasone at inducing matrix production by human bone marrow mesenchymal stem cell.短暂的机械加载与地塞米松诱导人骨髓间充质干细胞产生基质同样有效。
Eur Cell Mater. 2010 Jul 21;20:45-57. doi: 10.22203/ecm.v020a05.
2
Intrinsic extracellular matrix properties regulate stem cell differentiation.内在细胞外基质特性调节干细胞分化。
J Biomech. 2010 Jan 5;43(1):55-62. doi: 10.1016/j.jbiomech.2009.09.009. Epub 2009 Oct 2.
3
Tissue formation and vascularization in anatomically shaped human joint condyle ectopically in vivo.
电刺激对三维成骨细胞模型的影响。
Cells. 2025 Mar 8;14(6):396. doi: 10.3390/cells14060396.
4
Rapid osteoinduction of human adipose-derived stem cells grown on bioactive surfaces and stimulated by chemically modified media flow.在生物活性表面生长并受到化学修饰培养基流动刺激的人脂肪来源干细胞的快速骨诱导。
J Biol Eng. 2025 Mar 14;19(1):23. doi: 10.1186/s13036-025-00491-2.
5
Extrusion bioprinting: meeting the promise of human tissue biofabrication?挤出式生物打印：能否兑现人体组织生物制造的承诺？
Prog Biomed Eng (Bristol). 2025 Mar 11;7(2):023001. doi: 10.1088/2516-1091/adb254.
6
Bone Spheroid Development Under Flow Conditions with Mesenchymal Stem Cells and Human Umbilical Vein Endothelial Cells in a 3D Porous Hydrogel Supplemented with Hydroxyapatite.在添加羟基磷灰石的三维多孔水凝胶中，间充质干细胞和人脐静脉内皮细胞在流动条件下的骨球体发育。
Gels. 2024 Oct 18;10(10):666. doi: 10.3390/gels10100666.
7
The effect of brace treatment history on bone density in preoperative patients with adolescent idiopathic scoliosis (AIS) assessed by vertebral bone quality (VBQ) score: a retrospective propensity score-matched analysis.支具治疗史对基于椎体骨质量（VBQ）评分评估的青少年特发性脊柱侧凸（AIS）术前患者骨密度的影响：回顾性倾向评分匹配分析。
BMC Musculoskelet Disord. 2024 Aug 29;25(1):682. doi: 10.1186/s12891-024-07784-5.
8
Rapid prototyping of perfusion cell culture devices for three-dimensional imaging of mesenchymal stem cell deposition and proliferation.用于间充质干细胞沉积和增殖三维成像的灌注细胞培养装置的快速原型制作。
Heliyon. 2024 Jul 23;10(15):e35103. doi: 10.1016/j.heliyon.2024.e35103. eCollection 2024 Aug 15.
9
Stem Cells in Bone Tissue Engineering: Progress, Promises and Challenges.骨组织工程中的干细胞：进展、前景与挑战。
Stem Cell Rev Rep. 2024 Oct;20(7):1692-1731. doi: 10.1007/s12015-024-10738-y. Epub 2024 Jul 19.
10
Prediction of Bone Remodeling in Rat Caudal Vertebrae Based on Fluid-Solid Coupling Simulation.基于流固耦合模拟的大鼠尾骨骨重建预测。
Ann Biomed Eng. 2024 Nov;52(11):3009-3020. doi: 10.1007/s10439-024-03562-5. Epub 2024 Jun 28.
在体内异位构建具有解剖形状的人关节髁组织形成和血管化。
Tissue Eng Part A. 2009 Dec;15(12):3923-30. doi: 10.1089/ten.TEA.2008.0653.
4
Use of rapidly mineralising osteoblasts and short periods of mechanical loading to accelerate matrix maturation in 3D scaffolds.利用快速矿化的成骨细胞和短期机械加载来加速三维支架中的基质成熟。
Bone. 2009 May;44(5):822-9. doi: 10.1016/j.bone.2008.12.027. Epub 2009 Jan 14.
5
Responsiveness of human bone marrow stromal cells to shear stress.人骨髓基质细胞对剪切力的反应性。
J Tissue Eng Regen Med. 2009 Jun;3(4):302-9. doi: 10.1002/term.166.
6
Effect of fluid flow-induced shear stress on human mesenchymal stem cells: differential gene expression of IL1B and MAP3K8 in MAPK signaling.流体流动诱导的剪切应力对人间充质干细胞的影响：丝裂原活化蛋白激酶（MAPK）信号通路中白细胞介素1β（IL1B）和丝裂原活化蛋白激酶激酶激酶8（MAP3K8）的差异基因表达
Gene Expr Patterns. 2009 Jun;9(5):381-8. doi: 10.1016/j.gep.2009.01.001. Epub 2009 Jan 20.
7
Effect of low-frequency pulsatile flow on expression of osteoblastic genes by bone marrow stromal cells.低频脉动流对骨髓基质细胞成骨基因表达的影响。
Ann Biomed Eng. 2009 Mar;37(3):445-53. doi: 10.1007/s10439-008-9632-7. Epub 2009 Jan 8.
8
A comparative study of shear stresses in collagen-glycosaminoglycan and calcium phosphate scaffolds in bone tissue-engineering bioreactors.骨组织工程生物反应器中胶原蛋白-糖胺聚糖和磷酸钙支架剪切应力的比较研究。
Tissue Eng Part A. 2009 May;15(5):1141-9. doi: 10.1089/ten.tea.2008.0204.
9
Bone marrow-derived human mesenchymal stem cells become quiescent on soft substrates but remain responsive to chemical or mechanical stimuli.源自骨髓的人间充质干细胞在柔软基质上会进入静止状态，但对化学或机械刺激仍保持反应能力。
Tissue Eng Part A. 2009 Jan;15(1):147-54. doi: 10.1089/ten.tea.2007.0388.
10
Undifferentiated human mesenchymal stem cells (hMSCs) are highly sensitive to mechanical strain: transcriptionally controlled early osteo-chondrogenic response in vitro.未分化的人间充质干细胞（hMSCs）对机械应变高度敏感：体外转录控制的早期骨软骨生成反应。
Osteoarthritis Cartilage. 2007 Nov;15(11):1293-300. doi: 10.1016/j.joca.2007.04.002. Epub 2007 Oct 31.