切应力诱导人骨髓间充质干细胞的成骨分化。
Shear stress induces osteogenic differentiation of human mesenchymal stem cells.
机构信息
Department of Physiology & Biophysics, University of Illinois at Chicago, Chicago, IL 60612, USA.
出版信息
Regen Med. 2010 Sep;5(5):713-24. doi: 10.2217/rme.10.60.
Abstract
AIM
To determine whether fluid flow-induced shear stress affects the differentiation of bone marrow-derived human mesenchymal stem cells (hMSCs) into osteogenic cells.
MATERIALS & METHODS: hMSCs cultured with or without osteogenic differentiation medium were exposed to fluid flow-induced shear stress and analyzed for alkaline phosphatase activity and expression of osteogenic genes.
RESULTS
Immediately following shear stress, alkaline phosphatase activity in osteogenic medium was significantly increased. At days 4 and 8 of culture the mRNA expression of bone morphogenetic protein-2 and osteopontin was significantly higher in hMSCs subjected to shear stress than those cultured in static conditions. However, hMSCs cultured in osteogenic differentiation medium were less responsive in gene expression of alkaline phosphatase and bone morphogenetic protein-2.
CONCLUSION
These data demonstrate that shear stress stimulates hMSCs towards an osteoblastic phenotype in the absence of chemical induction, suggesting that certain mechanical stresses may serve as an alternative to chemical stimulation of stem cell differentiation.
摘要
目的
确定流体流动引起的切应力是否会影响骨髓来源的人骨髓间充质干细胞（hMSCs）向成骨细胞的分化。
材料与方法
将培养有或没有成骨分化培养基的 hMSCs 暴露于流体流动引起的切应力下，并分析碱性磷酸酶活性和成骨基因的表达。
结果
切应力后即刻，成骨培养基中的碱性磷酸酶活性显著增加。在培养的第 4 天和第 8 天，与静态培养条件相比，hMSCs 中的骨形态发生蛋白-2 和骨桥蛋白的 mRNA 表达明显更高。然而，在成骨分化培养基中培养的 hMSCs 在碱性磷酸酶和骨形态发生蛋白-2 的基因表达方面反应较弱。
结论
这些数据表明，在没有化学诱导的情况下，切应力刺激 hMSCs 向成骨细胞表型分化，这表明某些机械应力可能可作为干细胞分化的化学刺激的替代物。
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