Biochemistry Laboratory, Faculty of Science, University of Douala, P.O. Box 24157, Douala, Cameroon.
Institute of Agricultural Research for Development, Nko'olong Station, Kribi, Cameroon.
Curr Microbiol. 2023 Jan 3;80(2):63. doi: 10.1007/s00284-022-03165-0.
Improving the biochemical status of Spirulina platensis will enhance the functional properties of this microalgae. The present study investigated the effects of adding NaHCO to the culture medium on the growth rate and biochemical composition, particularly the coproduction of proteins, carbohydrates, and photosynthetic pigments of S. platensis. Spirulina platensis was grown in different NaHCO concentrations (0-16 g L). NaHCO positively affected the biomass production. The growth of S. platensis and biochemical compound content increased with an increase in the NaHCO concentration. The microalgae biomass grown on NaHCO also contained higher amounts of protein (64.20 ± 4.18% w w) and photosynthetic pigments (phycocyanin and chlorophyll a, b, and total). Protein productivity was especially enhanced by approximately 6-25% (from 0.006 ± 0.0030 to 0.025 ± 0.0031 mg L day) with the addition of NaHCO compared to the control. In contrast, the content of carbohydrates and antioxidant compounds (phenolic, polyphenol oxidase, and peroxidase activities) decreased with culture age and an increase in the NaHCO concentration. These results suggest that S. platensis uses NaHCO as a carbon source for photosynthesis, biomass production, and acts as a metabolic energy carrier toward the synthesis of proteins and photosynthetic pigments, which are more energy-consuming metabolites than carbohydrates. The addition of NaHCO to the culture media is a potentially useful strategy toward improving the protein and photosynthetic pigment productivity of S. platensis.
提高螺旋藻的生化状况将增强这种微藻的功能特性。本研究探讨了在培养基中添加碳酸氢钠对螺旋藻生长速度和生化成分的影响，特别是蛋白质、碳水化合物和光合色素的共生产。螺旋藻在不同的碳酸氢钠浓度（0-16 g/L）下生长。碳酸氢钠对生物量的产生有积极影响。随着碳酸氢钠浓度的增加，螺旋藻的生长和生化化合物含量增加。在碳酸氢钠上生长的微藻生物量还含有更高含量的蛋白质（64.20±4.18%w/w）和光合色素（藻蓝蛋白和叶绿素 a、b 和总）。与对照相比，添加碳酸氢钠可使蛋白质生产力提高约 6-25%（从 0.006±0.0030 增加到 0.025±0.0031 mg/L/天）。相比之下，碳水化合物和抗氧化化合物（酚类、多酚氧化酶和过氧化物酶活性）的含量随着培养时间的延长和碳酸氢钠浓度的增加而降低。这些结果表明，螺旋藻将碳酸氢钠用作光合作用、生物量生产的碳源，并作为代谢能量载体，有利于蛋白质和光合色素的合成，这些代谢物比碳水化合物更耗能。在培养基中添加碳酸氢钠是提高螺旋藻蛋白质和光合色素生产力的一种潜在有用策略。
