Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, Department of Pharmaceutical Analysis, College of Pharmacy, Yanbian University, Yanji 133002, Jilin Province, China.
Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, Department of Pharmaceutical Analysis, College of Pharmacy, Yanbian University, Yanji 133002, Jilin Province, China.
Food Res Int. 2023 Jan;163:112170. doi: 10.1016/j.foodres.2022.112170. Epub 2022 Nov 28.
α-dicarbonyl compounds (α-DCs) serve as potential biomarkers for oxidative stress-related diseases but are difficult to detect.To study the metabolism of carbonyl compounds, we developed a new mass spectrometry probe, 3-benzyl-2-oxo-4λ-thiazolidine-4-carbohydrazide (BOTC), containing hydrazyl groups for the targeted detection of carbonyl functional groups.In a novel approach, we used BOTC pre-column derivatization with ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) to simultaneously detect four kinds of α-DCs in red wine as well as in urine after drinking. The α-DCs were completely separated (R ≥ 0.9995), detection was sensitive (detection limit was 12.5-50 fmol), consistent (intraday and interday precision was 0.1-5.7 %), and efficient (average recoveries were 103.3-110.2 %). The method was applied to the analysis of α-DCs in different wines and the dynamic monitoring of transit and excretion in vivo after drinking. Our novel method provides a new strategy for the detection of α-dicarbonyl compounds in red wine and dicarbonyl compounds produced in oxidative stress-related diseases.
α-二羰基化合物（α-DCs）可作为氧化应激相关疾病的潜在生物标志物，但很难检测到。为了研究羰基化合物的代谢，我们开发了一种新的质谱探针，3-苄基-2-氧代-4λ-噻唑烷-4-甲酰肼（BOTC），其中含有酰肼基团，用于靶向检测羰基官能团。在一种新方法中，我们使用 BOTC 柱前衍生化与超高效液相色谱-串联质谱联用（UHPLC-MS/MS）同时检测红酒以及饮酒后尿液中的四种 α-DCs。α-DCs 完全分离（R≥0.9995），检测灵敏度高（检测限为 12.5-50 fmol），重现性好（日内和日间精密度为 0.1-5.7%），效率高（平均回收率为 103.3-110.2%）。该方法用于不同葡萄酒中 α-DCs 的分析以及饮酒后体内转运和排泄的动态监测。我们的新方法为红酒中 α-二羰基化合物以及氧化应激相关疾病中产生的二羰基化合物的检测提供了一种新策略。
