Luo Yanbo, Liu Yuwei, Li Xiangyu, Jiang Xingyi, Pang Yongqiang, Chen Di
China National Tobacco Quality Supervision and Test Center, Zhengzhou 450001, China.
Zhengzhou Research Base, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Zhengzhou University, Zhengzhou 450001, China.
Food Chem X. 2024 Sep 27;24:101858. doi: 10.1016/j.fochx.2024.101858. eCollection 2024 Dec 30.
α-Dicarbonyl compounds (α-DCs), formed during food heating and storage, are crucial for assessing food safety and quality. However, the low concentration, high reactivity, and absence of chromophores of α-DCs make their detection challenging, often requiring complex derivatization and extraction. This study developed a one-pot method for α-DCs that combines derivatization and magnetic solid-phase extraction. By mixing the sample, 2,3-diaminonaphthalene, and FeO/MWCNTs-OH in a vial, simultaneous derivatization and extraction are achieved. Derivatization converts α-DCs into hydrophobic products, facilitating their adsorption and enabling sensitive liquid chromatography-fluorescence detection. The introduction of the magnetic adsorbent allows phase separation to be easily achieved using an external magnet, simplifying and speeding up the process. The detection limits for six α-DCs (glyoxal, methylglyoxal, diacetyl, 2,3-pentanedione, D-glucosone, and 3-deoxyglucosone) were determined to be in the range of 0.4-3.5 nM. This rapid and convenient analytical approach was successfully applied to analyze α-DCs in juices, coffees, and tea beverages.
在食品加热和储存过程中形成的α-二羰基化合物（α-DCs）对于评估食品安全和质量至关重要。然而，α-DCs浓度低、反应活性高且缺乏发色团，这使得它们的检测具有挑战性，通常需要复杂的衍生化和萃取过程。本研究开发了一种用于α-DCs的一锅法，该方法将衍生化和磁性固相萃取相结合。通过在小瓶中混合样品、2,3-二氨基萘和FeO/MWCNTs-OH，实现了同时衍生化和萃取。衍生化将α-DCs转化为疏水性产物，便于其吸附，并实现灵敏的液相色谱-荧光检测。磁性吸附剂的引入使得使用外部磁铁即可轻松实现相分离，简化并加速了该过程。六种α-DCs（乙二醛、甲基乙二醛、双乙酰、2,3-戊二酮、D-葡萄糖酮和3-脱氧葡萄糖酮）的检测限确定为0.4-3.5 nM。这种快速便捷的分析方法已成功应用于分析果汁、咖啡和茶饮料中的α-DCs。
