Department of Environmental Science and Engineering, School of Energy and Environment, Wuxi Engineering Research Center of Taihu Lake Water Environment, Southeast University, Nanjing, Jiangsu 210096, China; Key Laboratory of Environmental Medicine Engineering, Ministry of Education, Southeast University, Nanjing, Jiangsu 210009, China.
School of Civil Engineering and Architecture, Zhejiang University of Science and Technology, Hangzhou, Zhejiang 310023, China.
Bioresour Technol. 2023 Feb;370:128567. doi: 10.1016/j.biortech.2022.128567. Epub 2022 Dec 31.
The effects and mechanisms of three N-acyl-homoserine lactones (AHLs) (C-HSL, C-HSL, and C-HSL) on responses of biological nitrogen removal (BNR) systems to zinc oxide nanoparticle (NP) shock were investigated. All three AHLs improved the NP-impaired ammonia oxidation rates by up to 50.0 % but inhibited the denitrification process via regulating nitrogen metabolism-related enzyme activities. C-HSL accelerated the catalase activity by 13.2 %, while C-HSL and C-HSL promoted the superoxide dismutase activity by 26.6 % and 18.4 %, respectively, to reduce reactive oxygen species levels. Besides, the enhancements of tryptophan protein and humic acid levels in tightly-bound extracellular polymeric substance by AHLs were vital for NP toxicity attenuation. The metabonomic analysis demonstrated that all three AHLs up-regulated the levels of lipid- and antioxidation-related metabolites to advance the system's resistance to NP shock. The "dual character" of AHLs emphasized the concernment of legitimately employing AHLs to alleviate NP stress for BNR systems.
研究了三种 N-酰基高丝氨酸内酯（AHLs）（C-HSL、C-HSL 和 C-HSL）对生物脱氮（BNR）系统对氧化锌纳米颗粒（NP）冲击响应的影响和机制。这三种 AHLs 都能将 NP 损伤的氨氧化速率提高高达 50.0%，但通过调节氮代谢相关酶的活性来抑制反硝化过程。C-HSL 使过氧化氢酶活性增加了 13.2%，而 C-HSL 和 C-HSL 则分别使超氧化物歧化酶活性增加了 26.6%和 18.4%，以降低活性氧水平。此外，AHLs 对紧密结合细胞外聚合物中色氨酸蛋白和腐殖酸水平的增强对于减轻 NP 毒性至关重要。代谢组学分析表明，这三种 AHLs 都上调了脂质和抗氧化相关代谢物的水平，以提高系统对 NP 冲击的抵抗力。AHLs 的“双重特性”强调了在生物脱氮系统中合理使用 AHLs 来缓解 NP 压力的问题。
