Han Hao, Li Jun, Zhang Jing, Peng Yongzhen, Li Yun, Zhang Kai, Zhang Yi, Wei Pengyuan, Luo Rong
National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China.
National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, PR China.
Bioresour Technol. 2021 Dec;341:125870. doi: 10.1016/j.biortech.2021.125870. Epub 2021 Sep 2.
A novel strategy based on quorum sense (QS) was proposed to improve the treatment performance of the partial denitrification/Anammox (PD/A) process at high loads by adding immobilized Pseudomonas sp. HFQ8, which could release high concentrations of N-butyryl-DL-homoserine lactone (C4-HSL), N-octanoyl-DL- homoserine lactone (C8-HSL) and N-decanoyl-DL-homoserine lactone (C10-HSL). The results showed that adding immobilized HFQ8 improved the sludge activity and settleability, contributing to higher nitrogen removal efficiency at the high nitrogen loading rate (NLR). It was proved that C4-HSL promoted the abundances of Thauera and Candidatus Kuenenia at NLR 1.68-2.52 kg N/(m·d), while C10-HSL promoted the abundance of Candidatus Brocadia. Besides, C8-HSL and C10-HSL played different regulation roles in the production of protein (PN) in tightly bound extracellular polymeric substances (TB-EPS) at different loads, improving the sludge settleability. This study provided a new way to improve the treatment performance of high-load PD/A processes.
提出了一种基于群体感应（QS）的新策略，通过添加能释放高浓度N-丁酰-DL-高丝氨酸内酯（C4-HSL）、N-辛酰-DL-高丝氨酸内酯（C8-HSL）和N-癸酰-DL-高丝氨酸内酯（C10-HSL）的固定化假单胞菌HFQ8，来提高高负荷下部分反硝化/厌氧氨氧化（PD/A）工艺的处理性能。结果表明，添加固定化HFQ8可提高污泥活性和沉降性能，有助于在高氮负荷率（NLR）下实现更高的氮去除效率。结果证明，在NLR为1.68-2.52 kg N/(m·d)时，C4-HSL促进了陶厄氏菌属和“库氏硝化菌”的丰度，而C10-HSL促进了“布罗卡德氏硝化菌”的丰度。此外，C8-HSL和C10-HSL在不同负荷下对紧密结合的胞外聚合物（TB-EPS）中蛋白质（PN）的产生发挥了不同的调节作用，改善了污泥沉降性能。本研究为提高高负荷PD/A工艺的处理性能提供了一种新方法。
