Comparative analysis of the molecular mechanism of inhibiting proliferation and migration in cervical cancer HeLa cell by curcumin and resveratrol.

Functional experiments indicated that curcumin displayed stronger inhibitory activity on the proliferation of cervical cancer HeLa cells, while resveratrol had a better inhibition effect on migration. Then, we compared the candidate target genes of curcumin and resveratrol in the treatment of cervical cancer through network pharmacology. GO enrichment results showed that curcumin exerted its anti-cervical cancer effect by regulating cell cycle mitosis, whereas resveratrol affected adhesion. Furthermore, the target genes were verified by molecular docking, qRT-PCR, and Western blot, the results revealed that curcumin and resveratrol significantly decreased the expression of CHEK1 and MAPK3, respectively. In conclusion, curcumin inhibited the proliferation of cervical cancer HeLa cells by specifically targeting CHEK1, while resveratrol specifically targeted MAPK3 to supress migration, and the combination of them can synergistically restrain the proliferation and migration of cervical cancer cells.