循环 miRNAs 在结直肠癌中的潜在诊断作用。
Potential diagnostic role of circulating MiRNAs in colorectal cancer.
机构信息
Biochemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt.
Biochemistry Department, Biotechnology Research Institute, High Throughput Molecular and Genetic laboratory, Central Laboratories Network and the Centers of Excellence, 68787National Research Centre, Cairo, Egypt.
出版信息
Int J Immunopathol Pharmacol. 2023 Jan-Dec;37:3946320221144565. doi: 10.1177/03946320221144565.
OBJECTIVES
Colorectal cancer (CRC) is the third most common and fourth most deadly cancer worldwide despite its various screening method. Thus, the search for novel and better markers is continuous. This study aimed to assess the combined expression levels of miR-133a, miR-574-3p, and miR-27a in early diagnosis of colorectal cancer in comparison to traditional tumor markers (CEA and CA19.9).
METHODS
miR-133a, miR-574-3p, and miR-27a were assessed in sera of 120 participants categorized into healthy control group ( = 20), benign group ( = 30) and malignant group ( = 70) using real-time PCR.
RESULTS
miR-133a, miR-574-3p, and miR-27a expressions showed significant difference among different staging, grading and tumor size of CRC. The sensitivities of the three miRNAs whether combined or individually used were better than routinely used tumor markers (CEA and CA19.9) leading to more accurate and faster diagnosis of CRC.
CONCLUSION
Synergetic detection of miRNA-133a, miRNA-574-3p, and miRNA-27a may serve as better noninvasive biomarkers with higher combined sensitivity for early diagnosis of CRC than individual detection of miRNAs.
目的
尽管有多种筛查方法，但结直肠癌（CRC）仍然是全球第三大常见癌症和第四大最致命癌症。因此，人们一直在寻找新的、更好的标志物。本研究旨在评估 miR-133a、miR-574-3p 和 miR-27a 的联合表达水平在结直肠癌早期诊断中的应用，与传统肿瘤标志物（CEA 和 CA19.9）相比。
方法
采用实时 PCR 法检测 120 例参与者（健康对照组 20 例、良性组 30 例和恶性组 70 例）血清中 miR-133a、miR-574-3p 和 miR-27a 的表达水平。
结果
miR-133a、miR-574-3p 和 miR-27a 的表达在 CRC 的不同分期、分级和肿瘤大小之间存在显著差异。三种 miRNA 无论是单独使用还是联合使用，其敏感性均优于常规使用的肿瘤标志物（CEA 和 CA19.9），从而更准确、更快地诊断 CRC。
结论
miR-133a、miR-574-3p 和 miR-27a 的联合检测可能作为更好的非侵入性生物标志物，与单独检测 miRNA 相比，具有更高的联合敏感性，可用于 CRC 的早期诊断。
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