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噬菌体-抗生素协同作用降低洋葱伯克霍尔德菌种群。

Phage-antibiotic synergy reduces Burkholderia cenocepacia population.

机构信息

Department of Cell and Developmental Biology, University of Illinois Urbana-Champaign, Urbana, IL, USA.

Southeast Denver Pediatrics, Denver, CO, USA.

出版信息

BMC Microbiol. 2023 Jan 5;23(1):2. doi: 10.1186/s12866-022-02738-0.

DOI:10.1186/s12866-022-02738-0
PMID:36600213
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9814465/
Abstract

BACKGROUND

Burkholderia cenocepacia is an opportunistic pathogen that can cause acute and chronic infections in patients with weakened immune systems and in patients with cystic fibrosis. B. cenocepacia is resistant to many antibiotics making treatment challenging. Consequently, there is a critical need for alternative strategies to treat B. cenocepacia infections such as using bacteriophages and/or bacteriophages with subinhibitory doses of antibiotic called phage-antibiotic synergy.

RESULTS

We isolated a bacteriophage, KP1, from raw sewage that infects B. cenocepacia. Its morphological characteristics indicate it belongs in the family Siphoviridae, it has a 52 Kb ds DNA genome, and it has a narrow host range. We determined it rescued infections in Lemna minor (duckweed) and moderately reduced bacterial populations in our artificial sputum medium model.

CONCLUSION

These results suggest that KP1 phage alone in the duckweed model or in combination with antibiotics in the ASMDM model improves the efficacy of reducing B. cenocepacia populations.

摘要

背景

洋葱伯克霍尔德菌是一种机会性病原体，可导致免疫系统较弱的患者和囊性纤维化患者发生急性和慢性感染。洋葱伯克霍尔德菌对许多抗生素具有耐药性，这使得治疗具有挑战性。因此，迫切需要替代策略来治疗洋葱伯克霍尔德菌感染，例如使用噬菌体和/或亚抑菌剂量抗生素的噬菌体，称为噬菌体-抗生素协同作用。

结果

我们从原污水中分离出一种感染洋葱伯克霍尔德菌的噬菌体 KP1。其形态特征表明它属于肌尾噬菌体科，基因组为 52 Kb dsDNA，宿主范围较窄。我们确定它可以挽救浮萍（浮萍）中的感染，并适度减少我们人工痰培养基模型中的细菌种群。

结论

这些结果表明，KP1 噬菌体单独在浮萍模型中或与 ASMDM 模型中的抗生素联合使用均可提高降低洋葱伯克霍尔德菌种群的疗效。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff7/9814465/8faa8e48b49d/12866_2022_2738_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff7/9814465/5813347fd6cf/12866_2022_2738_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff7/9814465/69272174270e/12866_2022_2738_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff7/9814465/fd56f827916a/12866_2022_2738_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff7/9814465/0eed479fd40c/12866_2022_2738_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff7/9814465/93fb60949d63/12866_2022_2738_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff7/9814465/e7111c6db181/12866_2022_2738_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff7/9814465/e4c294e15780/12866_2022_2738_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff7/9814465/8faa8e48b49d/12866_2022_2738_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff7/9814465/5813347fd6cf/12866_2022_2738_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff7/9814465/69272174270e/12866_2022_2738_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff7/9814465/fd56f827916a/12866_2022_2738_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff7/9814465/0eed479fd40c/12866_2022_2738_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff7/9814465/93fb60949d63/12866_2022_2738_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff7/9814465/e7111c6db181/12866_2022_2738_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff7/9814465/e4c294e15780/12866_2022_2738_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8ff7/9814465/8faa8e48b49d/12866_2022_2738_Fig8_HTML.jpg

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