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亚硒酸钠对语音假体上成熟混合细菌生物膜的抑制与去除作用

Inhibition and Removal of Mature Mixed-Bacteria Biofilms on Voice Prostheses by Sodium Selenite.

作者信息

Zhang Yongli, Niu Yanyan, Huo Hong, Wang Jian, Jin Xiaofeng, Yang Hua

机构信息

Peking Union Medical College, Chinese Academy of Medical Sciences, Peking Union Medical College Hospital, Department of Otolaryngology, Beijing, People's Republic of China.

Translational Medicine Center, Peking Union Medical College Hospital, Beijing, People's Republic of China.

出版信息

Infect Drug Resist. 2022 Dec 29;15:7799-7810. doi: 10.2147/IDR.S393434. eCollection 2022.

DOI:10.2147/IDR.S393434
PMID:36600950
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9807126/
Abstract

PURPOSE

Biofilms on voice prostheses are important factors shortening their service life. Sodium selenite has been used to prevent and treat various diseases. Whether sodium selenite can inhibit and remove mature biofilms on voice prostheses is still unknown.

METHODS

To verify the effects of sodium selenite on mature mixed-bacteria biofilms (, and ) on voice prostheses, we used quantitative and qualitative methods, eg, real-time fluorescence quantitative PCR, crystal violet staining, 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) (XTT) reduction assays, and scanning electron microscopy, to measure the effects of sodium selenite on the number of bacterial colonies, biofilm formation ability, metabolic activity, and ultrastructure in a model of mature mixed-bacteria biofilms on voice prostheses and validated the effects in vitro on mature biofilms on voice prostheses from patients.

RESULTS

When exploring the possible mechanism of biofilm inhibition and removal by sodium selenite, we found that it significantly inhibited and removed biofilms on voice prostheses and effectively destroyed the spatial structure of the biofilms. The inhibition and removal effects became more significant with increasing sodium selenite concentrations.

CONCLUSION

We demonstrated that sodium selenite can inhibit and remove biofilms of mature mixed strains on voice prostheses, providing a novel basis for treating patients' voice prosthesis biofilms.

摘要

目的

语音假体上的生物膜是缩短其使用寿命的重要因素。亚硒酸钠已被用于预防和治疗各种疾病。亚硒酸钠是否能抑制和去除语音假体上的成熟生物膜仍不清楚。

方法

为了验证亚硒酸钠对语音假体上成熟混合细菌生物膜（、和）的影响，我们使用了定量和定性方法，例如实时荧光定量PCR、结晶紫染色、2,3-双（2-甲氧基-4-硝基-5-磺基苯基）-2H-四唑-5-甲酰苯胺）（XTT）还原试验和扫描电子显微镜，以测量亚硒酸钠对语音假体上成熟混合细菌生物膜模型中细菌菌落数量、生物膜形成能力、代谢活性和超微结构的影响，并在体外验证了对患者语音假体上成熟生物膜的影响。

结果

在探索亚硒酸钠抑制和去除生物膜的可能机制时，我们发现它能显著抑制和去除语音假体上的生物膜，并有效破坏生物膜的空间结构。随着亚硒酸钠浓度的增加，抑制和去除效果变得更加显著。

结论

我们证明亚硒酸钠可以抑制和去除语音假体上成熟混合菌株的生物膜，为治疗患者语音假体生物膜提供了新的依据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57ce/9807126/bf8bc9d60726/IDR-15-7799-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57ce/9807126/9e8d296dbd67/IDR-15-7799-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57ce/9807126/075471750b53/IDR-15-7799-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57ce/9807126/330d8dcb8fa7/IDR-15-7799-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57ce/9807126/95f0e14e5fda/IDR-15-7799-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57ce/9807126/d5d1c70e70ce/IDR-15-7799-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57ce/9807126/52c8a00dc2a2/IDR-15-7799-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57ce/9807126/bf8bc9d60726/IDR-15-7799-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57ce/9807126/9e8d296dbd67/IDR-15-7799-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57ce/9807126/075471750b53/IDR-15-7799-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57ce/9807126/330d8dcb8fa7/IDR-15-7799-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57ce/9807126/95f0e14e5fda/IDR-15-7799-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57ce/9807126/d5d1c70e70ce/IDR-15-7799-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57ce/9807126/52c8a00dc2a2/IDR-15-7799-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57ce/9807126/bf8bc9d60726/IDR-15-7799-g0007.jpg

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